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  Nuclear pores dilate and constrict in cellulo

Zimmerli, C. E., Allegretti, M., Rantos, V., Goetz, S. K., Obarska-Kosinska, A., Zagoriy, I., et al. (2021). Nuclear pores dilate and constrict in cellulo. Science, 374(6573): eabd9776. doi:10.1126/science.abd9776.

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Zimmerli, Christian E.1, 2, 3, Author              
Allegretti, Matteo1, 3, Author              
Rantos, Vasileios4, 5, Author
Goetz, Sara K.2, 3, Author
Obarska-Kosinska, Agnieszka1, 5, Author              
Zagoriy, Ievgeniia3, Author
Halavatyi, Aliaksandr6, Author
Hummer, Gerhard7, 8, Author              
Mahamid, Julia3, Author
Kosinski, Jan3, 4, 5, Author
Beck, Martin1, 3, Author              
Affiliations:
1Department of Molecular Sociology, Max Planck Institute of Biophysics, Max Planck Society, ou_3040395              
2Collaboration for joint PhD degree between EMBL and Heidelberg University, Faculty of Biosciences, 69120 Heidelberg, Germany, ou_persistent22              
3Structural and Computational Biology Unit, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany, ou_persistent22              
4Centre for Structural Systems Biology (CSSB), Hamburg, Germany, ou_persistent22              
5EMBL Hamburg, Hamburg, Germany, ou_persistent22              
6Advanced Light Microscopy Facility, EMBL, Heidelberg, Germany, ou_persistent22              
7Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society, ou_2068292              
8Institute of Biophysics, Goethe University Frankfurt, Frankfurt am Main, Germany, ou_persistent22              

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 Abstract: In eukaryotic cells, nuclear pore complexes (NPCs) fuse the inner and outer nuclear membranes and mediate nucleocytoplasmic exchange. They are made of 30 different nucleoporins and form a cylindrical architecture around an aqueous central channel. This architecture is highly dynamic in space and time. Variations in NPC diameter have been reported, but the physiological circumstances and the molecular details remain unknown. Here we combined cryo-electron tomography (cryo-ET) with integrative structural modeling to capture a molecular movie of the respective large-scale conformational changes in cellulo. While NPCs of exponentially growing cells adopted a dilated conformation, they reversibly constricted upon cellular energy depletion or conditions of hypertonic osmotic stress. Our data point to a model where the nuclear envelope membrane tension is linked to the conformation of the NPC.

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Language(s): eng - English
 Dates: 2021-10-142020-07-242021-10-272021-11-112021-12-10
 Publication Status: Published in print
 Pages: 24
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.abd9776
BibTex Citekey: zimmerli_nuclear_nodate
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Title: Science
  Abbreviation : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: - Volume / Issue: 374 (6573) Sequence Number: eabd9776 Start / End Page: - Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1