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  The Arabidopsis electron-transfer flavoprotein:ubiquinone oxidoreductase is required during normal seed development and germination

da Fonseca-Pereira, P., Pham, P. A., Cavalcanti, J. H. F., Omena-Garcia, R. P., Barros, J. A. S., Rosado-Souza, L., et al. (2021). The Arabidopsis electron-transfer flavoprotein:ubiquinone oxidoreductase is required during normal seed development and germination. The Plant Journal. doi:10.1111/tpj.15566.

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Genre: Journal Article
Alternative Title : The Plant Journal

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 Creators:
da Fonseca-Pereira, P.1, Author              
Pham, P. A.1, Author              
Cavalcanti, João Henrique F.2, Author
Omena-Garcia, Rebeca P.2, Author
Barros, Jessica A. S.2, Author
Rosado-Souza, L.1, Author              
Vallarino, J. G.1, Author              
Mutwil, Marek2, Author
Avin-Wittenberg, Tamar2, Author
Nunes-Nesi, Adriano2, Author
Fernie, A. R.1, Author              
Araújo, Wagner L.2, Author
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2external, ou_persistent22              

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Free keywords: amino acid metabolism, ETF/ETFQO pathway, carbon partition, germination, primary metabolism, seed development
 Abstract: Abstract The importance of the alternative donation of electrons to the ubiquinol pool via the electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase (ETF/ETFQO) complex has been demonstrated. However, the functional significance of this pathway during seed development and germination remains to be elucidate. To assess the function of this pathway, we performed a detailed metabolic and transcription analyzes of Arabidopsis mutants to test the molecular consequences of a dysfunctional ETF/ETFQO pathway. We demonstrate that the disruption of this pathway compromises seed germination in the absence of an external Carbon-source and also impacts seed size and yield. Total protein and storage proteins content is reduced in dry seeds, whilst sucrose levels remain invariant. Seeds of ETFQO and related mutants were also characterized by an altered fatty acid composition. During seed development, lower levels of fatty acids and proteins accumulated in the etfqo-1 mutant as well as in the alternative electron donors, isovaleryl-CoA dehydrogenase (ivdh-1) and D-2-hydroxyglutarate dehydrogenase (d2hgdh1-2) mutant. Furthermore, the content of several amino acids was increased in etfqo-1 mutants during seed development, indicating that these mutants are not using such amino acids as alternative energy source for respiration. Transcriptome analysis revealed alterations in several genes involved in energy and hormonal metabolism. Our findings demonstrated that the alternative pathway of respiration mediated by the ETF/ETFQO complex affects seed germination and development by direct adjusting C storage during seed filling. These results provide a role for the pathway in the normal plant life cycle to complement its previously defined roles in response to abiotic stress.

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Language(s): eng - English
 Dates: 2021-11-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1111/tpj.15566
 Degree: -

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Title: The Plant Journal
  Other : Plant J.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0960-7412
CoNE: https://pure.mpg.de/cone/journals/resource/954925579095_1