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  Lactobacillus johnsonii BFE6154 ameliorates diet-induced hypercholesterolemia

Yoon, H., Lee, Y., Park, H., Kang, H.-J., Ji, Y., & Holzapfel, W. H. (2021). Lactobacillus johnsonii BFE6154 ameliorates diet-induced hypercholesterolemia. Probiotics and Antimicrobial Proteins. doi:10.1007/s12602-021-09859-4.

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 Creators:
Yoon, Hongsup1, Author              
Lee, Yuri, Author
Park, Haryung, Author
Kang, Hye-Ji, Author
Ji, Yosep, Author
Holzapfel, Wilhelm H., Author
Affiliations:
1Emeritus Group: Neuroimmunology / Wekerle, MPI of Neurobiology, Max Planck Society, ou_1113547              

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Free keywords: PICK C1-LIKE 1; FERMENTED MILK; CHOLESTEROL ABSORPTION; DOWN-REGULATION; MECHANISMS; PREVENTION; RECEPTOR; STRAINS; NPC1L1; SPP.Biotechnology & Applied Microbiology; Microbiology; Hypercholesterolemia; Probiotics; Lactobacillus johnsonii; Cholesterol uptake; Cholesterol efflux;
 Abstract: The functional characteristics of Lactobacillus johnsonii BFE6154, first isolated from Maasai traditional fermented milk, were previously identified in vitro, but its cholesterol-lowering properties have not been verified yet. In this study, we investigated the effect of L. johnsonii BFE6154 on cholesterol regulation and the mode of action. Stimulation of Caco-2 intestinal epithelial cells with L. johnsonii BFE6154 downregulated the gene expression of Niemann-Pick C1-like 1 (NPC1L1) through the activation of liver X receptor (LXR). Also, stimulation of HepG2 cells with the metabolites produced by L. johnsonii BFE6154 revealed an increase in the gene expression of low-density lipoprotein receptor (LDLR). Oral administration of L. johnsonii BFE6154 in mice receiving a high-fat and high-cholesterol diet (HFHCD), reduced total cholesterol and low-density lipoprotein-cholesterol (LDL) and increased high-density lipoprotein-cholesterol (HDL) in the blood, compared to the control. Diet-induced hypercholesterolemic mice receiving L. johnsonii BFE6154 showed a suppression of cholesterol absorption under the control of NPC1L1 in the intestine. Furthermore, L. johnsonii BFE6154 consumption ameliorated the hepatic cholesterol level and LDLR expression, which was reduced by HFHCD. These molecular modulations led to the increase of cholesterol excretion and the decrease of cholesterol levels in the feces and liver, respectively. Taken together, these results suggest that L. johnsonii BFE6154 may protect against diet-induced hypercholesterolemia through the regulation of cholesterol metabolism in the intestine and liver.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Probiotics and Antimicrobial Proteins
Source Genre: Journal
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Publ. Info: ONE NEW YORK PLAZA, SUITE 4600, NEW YORK, NY, UNITED STATES : SPRINGER
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1867-1306