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Free keywords:
FATTY LIVER-DISEASE; PLASMA PROTEOME DATABASE; TOTAL LIPID EXTRACTION;
MASS-SPECTROMETRY; NONALCOHOLIC STEATOHEPATITIS; AMINO-ACID; RNA-SEQ;
METABOLOMICS; SERUM; CHROMATOGRAPHYGeneral & Internal Medicine; biomarker discovery; liver disease; machine learning; multi-omics;
NAFLD; systems biology;
Abstract:
Non-alcoholic steatohepatitis (NASH) is a chronic liver disease affecting up to 6.5% of the general population. There is no simple definition of NASH, and the molecular mechanism underlying disease pathogenesis remains elusive. Studies applying single omics technologies have enabled a better understanding of the molecular profiles associated with steatosis and hepatic inflammation-the commonly accepted histologic features for diagnosing NASH, as well as the discovery of novel candidate biomarkers. Multi-omics analysis holds great potential to uncover new insights into disease mechanism through integrating multiple layers of molecular information. Despite the technical and computational challenges associated with such efforts, a few pioneering studies have successfully applied multi-omics technologies to investigate NASH. Here, we review the most recent technological developments in mass spectrometry (MS)-based proteomics, metabolomics, and lipidomics. We summarize multi-omics studies and emerging omics biomarkers in NASH and highlight the biological insights gained through these integrated analyses.</p>
