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  Effect of co-presentation of adhesive ligands and short hyaluronan on lymphendothelial cells

Antoni, C. H., McDuffie, Y., Bauer, J., Sleeman, J. P., & Boehm, H. (2018). Effect of co-presentation of adhesive ligands and short hyaluronan on lymphendothelial cells. Frontiers in Bioengineering and Biotechnology, 6: 25, pp. 1-7. doi:10.3389/fbioe.2018.00025.

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 Creators:
Antoni, Christiane H.1, 2, Author           
McDuffie, Yvonne1, 2, Author           
Bauer, Jochen, Author
Sleeman, Jonathan P., Author
Boehm, Heike1, 2, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              
2Biophysical Chemistry, Institute of Pysical Chemistry, University of Heidelberg, 69120 Heidelberg, Germany, ou_persistent22              

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Free keywords: hyaluronan, lymphangiogenesis, extracellular matrix mimetic, lymphendothelial cells, bioactive interface
 Abstract: Controlled activation of lymphangiogenesis through functional biomaterials represents a promising approach to support wound healing after surgical procedures, yet remains a challenge. In a synthetic biological approach, we therefore set out to mimic the basal microenvironment of human primary dermal lymphatic endothelial cells (LECs) during lymphangiogenesis. As the extracellular matrix component hyaluronan (HA) regulates lymphangiogenesis, we designed a bifunctional surface in which adhesive peptide ligands and short HA oligosaccharides (sHA) tethered to nanoparticles are copresented to the basal side of LECs in a controlled, concentration-dependent manner. Exposure of LECs to sHA in solution to mimic luminal stimulation of the cells did not result in modified metabolic activity. However, LECs grown on the bifunctional adhesive surfaces showed a biphasic change in metabolic activity, with increased metabolic activity being observed in response to increasing nanoparticle densities up to a maximum of 540 particles/μm2. Thus, interfaces that concomitantly present adhesive ligands and sHA can stimulate LEC metabolism and might be able to trigger lymphangiogenesis.

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Language(s): eng - English
 Dates: 2017-12-132018-03-052018-03-23
 Publication Status: Published online
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3389/fbioe.2018.00025
 Degree: -

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Title: Frontiers in Bioengineering and Biotechnology
  Abbreviation : Front. Bioeng. Biotechnol.
Source Genre: Journal
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Publ. Info: Lausanne : Frontiers Media
Pages: - Volume / Issue: 6 Sequence Number: 25 Start / End Page: 1 - 7 Identifier: ISSN: 2296-4185
CoNE: https://pure.mpg.de/cone/journals/resource/2296-4185