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  A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection

Xiao, T., Roser, T., Staub, W., & Baier, H. (2005). A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection. Development, 132(13), 2955-2967. doi:10.1242/dev.01861.

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 Creators:
Xiao, T., Author
Roser, T., Author
Staub, W., Author
Baier, Herwig1, Author           
Affiliations:
1University of California, San Francisco, U.S.A., ou_persistent22              

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Free keywords: retinal ganglion cell tectum transgenic mutant axon guidance brn3c pou4f3 zebrafish retinal ganglion-cells lamina-specific connectivity chick optic tectum visual-system axon guidance adhesion molecule in-vivo developmental expression cadherin expression dorsoventral axis Developmental Biology
 Abstract: The retinotectal projection is a premier model system for the investigation of molecular mechanisms that underlie axon pathfinding and map formation. Other important features, such as the laminar targeting of retinal axons, the control of axon fasciculation and the intrinsic organization of the tectal neuropil, have been less accessible to investigation. In order to visualize these processes in vivo, we generated a transgenic zebrafish line expressing membrane-targeted GFP under control of the brn3c promoter/enhancer. The GFP reporter labels a distinct subset of retinal ganglion cells (RGCs), which project mainly into one of the four retinorecipient layers of the tectum and into a small subset of the extratectal arborization fields. In this transgenic line, we carried out an ENU-mutagenesis screen by scoring live zebrafish larvae for anatomical phenotypes. Thirteen recessive mutations in 12 genes were discovered. In one mutant, ddl, the majority of RGCs fail to differentiate. Three of the mutations, vrt, late and tard, delay the orderly ingrowth of retinal axons into the tectum. Two alleles of drg disrupt the layer-specific targeting of retinal axons. Three genes,fuzz, beyo and brek, are required for confinement of the tectal neuropil. Fasciculation within the optic tract and adhesion within the tectal neuropil are regulated by vrt, coma, bluk, clew and blin. The mutated genes are predicted to encode molecules essential for building the intricate neural architecture of the visual system.

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Language(s): eng - English
 Dates: 2005
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000231050700003
DOI: 10.1242/dev.01861
ISSN: 0950-1991
 Degree: -

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Title: Development
  Other : Development
Source Genre: Journal
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Publ. Info: Cambridge, Cambridgeshire : Company of Biologists
Pages: - Volume / Issue: 132 (13) Sequence Number: - Start / End Page: 2955 - 2967 Identifier: ISSN: 0950-1991
CoNE: https://pure.mpg.de/cone/journals/resource/954927546241