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  Rational engineering of Luminiphilus syltensis ( R)-selective amine transaminase for the acceptance of bulky substrates

Konia, E., Chatzicharalampous, K., Drakonaki, A., Münke, C., Ermler, U., Tsiotis, G., et al. (2021). Rational engineering of Luminiphilus syltensis ( R)-selective amine transaminase for the acceptance of bulky substrates. Chemical Communications, 57(96), 12948-12951. doi:10.1039/D1CC04664K.

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 Creators:
Konia, Eleni1, Author
Chatzicharalampous, Konstantinos1, Author
Drakonaki, Athina1, Author
Münke, Cornelia2, Author           
Ermler, Ulrich2, Author           
Tsiotis, Georgios1, Author
Pavlidis, Ioannis V.1, Author
Affiliations:
1Department of Chemistry, University of Crete, Voutes University Campus, Heraklion, Greece , ou_persistent22              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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 Abstract: Despite the plethora of information on (S)-selective amine transaminases, the (R)-selective ones are still not well-studied; only a few structures are known to date, and their substrate scope is limited, apart from a few stellar works in the field. Herein, the structure of Luminiphilus syltensis (R)-selective amine transaminase is elucidated to facilitate engineering towards variants active on bulkier substrates. The V37A variant led to increased activity towards 1-phenylpropylamine and to activity against 1-butylamine. In contrast, the S248 and T249 positions, located on the β-turn in the P-pocket, seem crucial for maintaining the activity of the enzyme.

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Language(s): eng - English
 Dates: 2021-08-212021-11-112021-11-112021-12-14
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1039/D1CC04664K
PMID: 34806715
 Degree: -

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Title: Chemical Communications
  Abbreviation : Chem. Commun.
Source Genre: Journal
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Publ. Info: Cambridge, UK : Royal Society of Chemistry
Pages: - Volume / Issue: 57 (96) Sequence Number: - Start / End Page: 12948 - 12951 Identifier: ISSN: 1359-7345
CoNE: https://pure.mpg.de/cone/journals/resource/954928495413