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  Two-photon 3D laser printing inside synthetic cells

Abele, T., Messer, T., Jahnke, K., Hippler, M., Bastmeyer, M., Wegener, M., et al. (2021). Two-photon 3D laser printing inside synthetic cells. Advanced Materials, 2106709, pp. 1-16. doi:10.1002/adma.202106709.

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 Creators:
Abele, Tobias1, Author              
Messer, Tobias, Author
Jahnke, Kevin1, Author              
Hippler, Marc, Author
Bastmeyer, Martin, Author
Wegener, Martin, Author
Göpfrich, Kerstin1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: 3D laser printing; PEGDA hydrogel; additive manufacturing; bottom-up synthetic biology; direct laser writing; giant unilamellar lipid vesicles (GUVs); transmembrane pore
 Abstract: Towards the ambitious goal of manufacturing synthetic cells from the bottom up, various cellular components have already been reconstituted inside of lipid vesicles. However, the deterministic positioning of these components inside the compartment has remained elusive. Here, by using two-photon 3D laser printing, 2D and 3D hydrogel architectures were manufactured with high precision and nearly arbitrary shape inside of preformed giant unilamellar lipid vesicles (GUVs). The required water-soluble photoresist is brought into the GUVs by diffusion in a single mixing step. Crucially, femtosecond two-photon printing inside the compartment does not destroy the GUVs. Beyond this proof-of-principle demonstration, early functional architectures were realized. In particular, a transmembrane structure acting as a pore was 3D printed, thereby allowing for the transport of biological cargo, including DNA, into the synthetic compartment. These experiments show that two-photon 3D laser microprinting can be an important addition to the existing toolbox of synthetic biology. This article is protected by copyright. All rights reserved.

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Language(s): eng - English
 Dates: 2021-11-20
 Publication Status: Published online
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adma.202106709
 Degree: -

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Title: Advanced Materials
  Other : Adv. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: 2106709 Start / End Page: 1 - 16 Identifier: ISSN: 0935-9648
CoNE: https://pure.mpg.de/cone/journals/resource/954925570855