English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
 DetailsSummary
  Two-photon 3D laser printing inside synthetic cells

Abele, T., Messer, T., Jahnke, K., Hippler, M., Bastmeyer, M., Wegener, M., et al. (2021). Two-photon 3D laser printing inside synthetic cells. Advanced Materials, 2106709, pp. 1-16. doi:10.1002/adma.202106709.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0009-8585-A Version Permalink: https://hdl.handle.net/21.11116/0000-0009-8586-9
Genre: Journal Article

Files

show Files
hide Files
:
AdvMaterials_epub_2021_2106709.pdf (Any fulltext), 7MB
 
File Permalink:
-
Name:
AdvMaterials_epub_2021_2106709.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvMaterials_epub_2021_2106709_Suppl1.pdf (Supplementary material), 9MB
 
File Permalink:
-
Name:
AdvMaterials_epub_2021_2106709_Suppl1.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvMaterials_epub_2021_2106709_Suppl2.mp4 (Supplementary material), 6MB
 
File Permalink:
-
Name:
AdvMaterials_epub_2021_2106709_Suppl2.mp4
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvMaterials_epub_2021_2106709_Suppl3.mp4 (Supplementary material), 9MB
 
File Permalink:
-
Name:
AdvMaterials_epub_2021_2106709_Suppl3.mp4
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://doi.org/10.1002/adma.202106709 (Any fulltext)
Description:
-
OA-Status:
Not specified
Locator:
https://onlinelibrary.wiley.com/doi/epdf/10.1002/adma.202106709 (Any fulltext)
Description:
-
OA-Status:
Not specified
Locator:
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadma.202106709&file=adma202106709-sup-0001-SuppMat.pdf (Supplementary material)
Description:
-
OA-Status:
Not specified
Locator:
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadma.202106709&file=adma202106709-sup-0002-MovieS1.mp4 (Supplementary material)
Description:
-
OA-Status:
Not specified
Locator:
https://onlinelibrary.wiley.com/action/downloadSupplement?doi=10.1002%2Fadma.202106709&file=adma202106709-sup-0003-MovieS2.mp4 (Supplementary material)
Description:
-
OA-Status:
Not specified

Creators

show
hide
 Creators:
Abele, Tobias1, Author           
Messer, Tobias, Author
Jahnke, Kevin1, Author           
Hippler, Marc, Author
Bastmeyer, Martin, Author
Wegener, Martin, Author
Göpfrich, Kerstin1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: 3D laser printing; PEGDA hydrogel; additive manufacturing; bottom-up synthetic biology; direct laser writing; giant unilamellar lipid vesicles (GUVs); transmembrane pore
 Abstract: Towards the ambitious goal of manufacturing synthetic cells from the bottom up, various cellular components have already been reconstituted inside of lipid vesicles. However, the deterministic positioning of these components inside the compartment has remained elusive. Here, by using two-photon 3D laser printing, 2D and 3D hydrogel architectures were manufactured with high precision and nearly arbitrary shape inside of preformed giant unilamellar lipid vesicles (GUVs). The required water-soluble photoresist is brought into the GUVs by diffusion in a single mixing step. Crucially, femtosecond two-photon printing inside the compartment does not destroy the GUVs. Beyond this proof-of-principle demonstration, early functional architectures were realized. In particular, a transmembrane structure acting as a pore was 3D printed, thereby allowing for the transport of biological cargo, including DNA, into the synthetic compartment. These experiments show that two-photon 3D laser microprinting can be an important addition to the existing toolbox of synthetic biology. This article is protected by copyright. All rights reserved.

Details

show
hide
Language(s): eng - English
 Dates: 2021-11-20
 Publication Status: Published online
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adma.202106709
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Advanced Materials
  Other : Adv. Mater.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: - Sequence Number: 2106709 Start / End Page: 1 - 16 Identifier: ISSN: 0935-9648
CoNE: https://pure.mpg.de/cone/journals/resource/954925570855