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  Targeting neural circuitry in zebrafish using GAL4 enhancer trapping

Scott, E. K., Mason, L., Arrenberg, A. B., Ziv, L., Gosse, N. J., Xiao, T., et al. (2007). Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nature Methods, 4(4), 323-326. doi:10.1038/nmeth1033.

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 Creators:
Scott, E. K., Author
Mason, L., Author
Arrenberg, A. B., Author
Ziv, L., Author
Gosse, N. J., Author
Xiao, T., Author
Chi, N. C., Author
Asakawa, K., Author
Kawakami, K., Author
Baier, Herwig1, Author           
Affiliations:
1University of California, San Francisco, U.S.A., ou_persistent22              

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Free keywords: gene-expression in-vivo transposon Biochemistry & Molecular Biology
 Abstract: We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.

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Language(s): eng - English
 Dates: 2007
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000245584900012
DOI: 10.1038/nmeth1033
ISSN: 1548-7091
 Degree: -

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Title: Nature Methods
  Other : Nature Methods
Source Genre: Journal
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Affiliations:
Publ. Info: New York, NY : Nature Publishing Group
Pages: - Volume / Issue: 4 (4) Sequence Number: - Start / End Page: 323 - 326 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556