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  Fusion-induced growth of biomimetic polymersomes : behavior of poly(dimethylsiloxane)-poly(ethylene oxide) vesicles in saline solutions under high agitation

Marušič, N., Zhao, Z., Otrin, L., Dimova, R., Ivanov, I., & Sundmacher, K. (2022). Fusion-induced growth of biomimetic polymersomes: behavior of poly(dimethylsiloxane)-poly(ethylene oxide) vesicles in saline solutions under high agitation. Macromolecular Rapid Communications, 43(5): 2100712. doi:10.1002/marc.202100712.

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Marušič, Nika, Author
Zhao, Ziliang1, Author           
Otrin, Lado, Author
Dimova, Rumiana2, Author                 
Ivanov, Ivan, Author
Sundmacher, Kai, Author
Affiliations:
1Rumiana Dimova, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863328              
2Rumiana Dimova, Kolloidchemie, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3360040              

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Free keywords: biomimetic, fusion, microfluidics, polymersomes, vesicles
 Abstract: Giant unilamellar vesicles serve as membrane models and primitive mockups of natural cells. With respect to the latter use, amphiphilic polymers can be used to replace phospholipids in order to introduce certain favorable properties, ultimately allowing for the creation of truly synthetic cells. These new properties also enable the employment of new preparation procedures that are incompatible with the natural amphiphiles. Whereas the growth of lipid compartments to micrometer dimensions has been well established, growth of their synthetic analogs remains underexplored. Here, we investigate in detail the influence of experimental parameters like salt type/concentration and magnitude of agitation on the fusion of nanometer-sized vesicles made of poly(dimethylsiloxane)-poly(ethylene oxide) graft copolymer (PDMS-g-PEO). To this end, we employ dynamic light scattering, microscopy and membrane mixing assays, and analyze the process at different time and length scales. We use this optimized method as an easy tool to obtain giant vesicles, equipped with membrane and cytosolic biomachinery, in the presence of salts at physiological concentrations.

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Language(s): eng - English
 Dates: 2021-11-252022
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/marc.202100712
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Title: Macromolecular Rapid Communications
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 43 (5) Sequence Number: 2100712 Start / End Page: - Identifier: ISSN: 1022-1336