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  Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

Kage, D., Heinrich, K., von Volkmann, K., Kirsch, J., Feher, K., Giesecke-Thiel, C., et al. (2021). Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification. Communications Biology, 4: 1144. doi:10.1038/s42003-021-02664-3.

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© The Author(s) 2021

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 Urheber:
Kage, Daniel, Autor
Heinrich, Kerstin , Autor
von Volkmann, Konrad , Autor
Kirsch, Jenny, Autor
Feher, Kristen , Autor
Giesecke-Thiel, Claudia1, Autor           
Kaiser , Toralf, Autor
Affiliations:
1Flow Cytometry Facility (Head: Claudia Giesecke-Thiel), Scientific Service (Head: Christoph Krukenkamp), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_3039333              

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 Zusammenfassung: Flow cytometers are robust and ubiquitous tools of biomedical research, as they enable high-
throughput fluorescence-based multi-parametric analysis and sorting of single cells. How-
ever, analysis is often constrained by the availability of detection reagents or functional

changes of cells caused by fluorescent staining. Here, we introduce MAPS-FC (multi-angle
pulse shape flow cytometry), an approach that measures angle- and time-resolved scattered
light for high-throughput cell characterization to circumvent the constraints of conventional

flow cytometry. In order to derive cell-specific properties from the acquired pulse shapes, we
developed a data analysis procedure based on wavelet transform and k-means clustering. We

analyzed cell cycle stages of Jurkat and HEK293 cells by MAPS-FC and were able to assign

cells to the G1, S, and G2/M phases without the need for fluorescent labeling. The results
were validated by DNA staining and by sorting and re-analysis of isolated G1, S, and G2/M

populations. Our results demonstrate that MAPS-FC can be used to determine cell properties

that are otherwise only accessible by invasive labeling. This approach is technically com-

patible with conventional flow cytometers and paves the way for label-free cell sorting.

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Sprache(n): eng - English
 Datum: 2021-09-102021-09-30
 Publikationsstatus: Online veröffentlicht
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: DOI: 10.1038/s42003-021-02664-3
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Titel: Communications Biology
Genre der Quelle: Zeitschrift
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Affiliations:
Ort, Verlag, Ausgabe: London : Springer Nature
Seiten: - Band / Heft: 4 Artikelnummer: 1144 Start- / Endseite: - Identifikator: ISSN: 2399-3642
CoNE: https://pure.mpg.de/cone/journals/resource/2399-3642