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  Histone variant H2A.Z regulates zygotic genome activation

Ibarra-Morales, D., Rauer, M., Quarato, P., Rabbani, L., Zenk, F., Schulte-Sasse, M., et al. (2021). Histone variant H2A.Z regulates zygotic genome activation. Nature Communications, 12: 7002. doi:10.1038/s41467-021-27125-7.

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Ibarra-Morales et al. 2021.pdf (Publisher version), 4MB
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2021
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The Author(s) 2021

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 Creators:
Ibarra-Morales, Dafne1, Author
Rauer, Michael2, Author
Quarato, Piergiuseppe3, Author
Rabbani, Leily2, Author
Zenk, Fides1, Author
Schulte-Sasse, Mariana1, Author
Cardamone, Francesco1, Author
Gomez-Auli, Alejandro2, Author
Cecere, Germano3, Author
Iovino, Nicola1, Author              
Affiliations:
1Department of Chromatin Regulation, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243643              
2Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_persistent22              
3External Organizations, ou_persistent22              

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 Abstract: During embryogenesis, the genome shifts from transcriptionally quiescent to extensively active in a process known as Zygotic Genome Activation (ZGA). In Drosophila, the pioneer factor Zelda is known to be essential for the progression of development; still, it regulates the activation of only a small subset of genes at ZGA. However, thousands of genes do not require Zelda, suggesting that other mechanisms exist. By conducting GRO-seq, HiC and ChIP-seq in Drosophila embryos, we demonstrate that up to 65% of zygotically activated genes are enriched for the histone variant H2A.Z. H2A.Z enrichment precedes ZGA and RNA Polymerase II loading onto chromatin. In vivo knockdown of maternally contributed Domino, a histone chaperone and ATPase, reduces H2A.Z deposition at transcription start sites, causes global downregulation of housekeeping genes at ZGA, and compromises the establishment of the 3D chromatin structure. We infer that H2A.Z is essential for the de novo establishment of transcriptional programs during ZGA via chromatin reorganization.

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Language(s): eng - English
 Dates: 2021-12-01
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-27125-7
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 Sequence Number: 7002 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723