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  Towards Visual Proteomics at High Resolution

Bäuerlein, F. J. B., & Baumeister, W. (2021). Towards Visual Proteomics at High Resolution. Journal of Molecular Biology, 433(20): 167187. doi:10.1016/j.jmb.2021.167187.

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1-s2.0-S0022283621004204-main.pdf (Publisher version), 4MB
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© 2021 The Authors.

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 Creators:
Bäuerlein, Felix J. B.1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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Free keywords: BEAM-INDUCED MOTION; CORRELATIVE SUPERRESOLUTION FLUORESCENCE; MULTICOLOR LOCALIZATION MICROSCOPY; CRYOELECTRON MICROSCOPY; ELECTRON-MICROSCOPY; MOLECULAR SOCIOLOGY; CRYO-FLUORESCENCE; PHASE PLATE; TOMOGRAPHY; CELLSBiochemistry & Molecular Biology; cryo electron tomography; cryo-FIB; cryo-CLEM; super-resolution light microscopy; machine learning;
 Abstract: Traditionally, structural biologists approach the complexity of cellular proteomes in a reductionist manner. Proteomes are fractionated, their molecular components purified and studied one-by-one using the experimental methods for structure determination at their disposal. Visual proteomics aims at obtaining a holistic picture of cellular proteomes by studying them in situ, ideally in unperturbed cellular environments. The method that enables doing this at highest resolution is cryo-electron tomography. It allows to visualize cellular landscapes with molecular resolution generating maps or atlases revealing the interaction networks which underlie cellular functions in health and in disease states. Current implementations of cryo ET do not yet realize the full potential of the method in terms of resolution and interpretability. To this end, further improvements in technology and methodology are needed. This review describes the state of the art as well as measures which we expect will help overcoming current limitations. (C) 2021 Published by Elsevier Ltd.

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Language(s): eng - English
 Dates: 2021-082021-10
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000713305500015
DOI: 10.1016/j.jmb.2021.167187
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Title: Journal of Molecular Biology
  Other : JMB
  Abbreviation : J. Mol. Biol.
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: 433 (20) Sequence Number: 167187 Start / End Page: - Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/0022-2836