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  Proton detected solid-state NMR of membrane proteins at 28 Tesla (1.2 GHz) and 100 kHz magic-angle spinning

Nimerovsky, E., Tekwani Movellan, K., Zhang, X. C., Forster, M. C., Najbauer, E., Xue, K., et al. (2021). Proton detected solid-state NMR of membrane proteins at 28 Tesla (1.2 GHz) and 100 kHz magic-angle spinning. Biomolecules, 11(5): 752. doi:10.3390/biom11050752.

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Nimerovsky, E.1, Autor           
Tekwani Movellan, K.2, Autor           
Zhang, X. C., Autor
Forster, M. C., Autor
Najbauer, E.2, Autor           
Xue, K.2, Autor           
Dervisoglu, R.2, Autor           
Giller, K.3, Autor           
Griesinger, C.4, Autor                 
Becker, S.4, Autor           
Andreas, L. B.2, Autor           
Affiliations:
1Department of NMR Based Structural Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_578567              
2Research Group of Solid State NMR Spectroscopy-2, MPI for Biophysical Chemistry, Max Planck Society, ou_2396693              
3Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              
4Department of NMR Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

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Schlagwörter: magic-angle spinning; solid-state NMR; membrane protein; beta barrel; transmembrane; proton detection; high magnetic field
 Zusammenfassung: The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz. For magic-angle spinning (MAS) NMR, this is expected to improve resolution, provided the sample preparation results in homogeneous broadening. We compare two-dimensional (2D) proton detected MAS NMR spectra of four membrane proteins at 950 and 1200 MHz. We find a consistent improvement in resolution that scales superlinearly with the increase in magnetic field for three of the four examples. In 3D and 4D spectra, which are now routinely acquired, this improvement indicates the ability to resolve at least 2 and 2.5 times as many signals, respectively.

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Sprache(n): eng - English
 Datum: 2021-05-18
 Publikationsstatus: Online veröffentlicht
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.3390/biom11050752
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Titel: Biomolecules
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 11 (5) Artikelnummer: 752 Start- / Endseite: - Identifikator: -