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  Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes

Qutbuddin, Y., Krohn, J.-H., Brüggenthies, G. A., Stein, J., Gavrilovic, S., Stehr, F., et al. (2021). Design Features to Accelerate the Higher-Order Assembly of DNA Origami on Membranes. The Journal of Physical Chemistry B, 125, 3181-13191. doi:10.1021/acs.jpcb.1c07694.

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© 2021 The Authors. Open access funded by Max Planck Society.
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 Creators:
Qutbuddin, Yusuf1, Author
Krohn, Jan-Hagen1, Author
Brüggenthies, Gereon A. 1, Author
Stein, Johannes1, Author              
Gavrilovic, Svetozar 1, Author
Stehr, Florian1, Author              
Schwille, Petra1, Author              
Affiliations:
1Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565169              

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Free keywords: Chemical structure, Superstructures, Scaffolds, Nucleic acid structure, DNA origami
 Abstract: Nanotechnology often exploits DNA origami nanostructures assembled into even larger superstructures up to micrometer sizes with nanometer shape precision. However, large-scale assembly of such structures is very time-consuming. Here, we investigated the efficiency of superstructure assembly on surfaces using indirect cross-linking through low-complexity connector strands binding staple strand extensions, instead of connector strands binding to scaffold loops. Using single-molecule imaging techniques, including fluorescence microscopy and atomic force microscopy, we show that low sequence complexity connector strands allow formation of DNA origami superstructures on lipid membranes, with an order-of-magnitude enhancement in the assembly speed of superstructures. A number of effects, including suppression of DNA hairpin formation, high local effective binding site concentration, and multivalency are proposed to contribute to the acceleration. Thus, the use of low-complexity sequences for DNA origami higher-order assembly offers a very simple but efficient way of improving throughput in DNA origami design.

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 Dates: 2021-08-312021-11-24
 Publication Status: Published online
 Pages: -
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 Table of Contents: Published as part of The Journal of Physical Chemistry virtual special issue “W. E. Moerner Festschrift”.
 Rev. Type: -
 Identifiers: DOI: 10.1021/acs.jpcb.1c07694
 Degree: -

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Project name : BIOMOLMACS Marie Skłodowska-Curie grant agreement (Y.Q.)
Grant ID : 859416
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)
Project name : EXC-2094–390783311
Grant ID : -
Funding program : Germany’s Excellence Strategy
Funding organization : Deutsche Forschungsgemeinschaft (DFG, German Research Foundation)

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Title: The Journal of Physical Chemistry B
  Abbreviation : J. Phys. Chem. B
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 125 Sequence Number: - Start / End Page: 3181 - 13191 Identifier: ISSN: 1520-6106
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000293370_1