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  An environmental control box for serial crystallography enables multi-dimensional experiments

Mehrabi, P., von Stetten, D., Leimkohl, J.-P., Tellkamp, F., & Schulz, E.-C. (2021). An environmental control box for serial crystallography enables multi-dimensional experiments. doi:10.1101/2021.11.07.467596.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-9940-2 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-EF76-2
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2021.11.07.467596v2.full.pdf (Preprint), 7MB
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 Creators:
Mehrabi, P.1, 2, Author           
von Stetten, D.3, Author
Leimkohl, J.-P.4, Author           
Tellkamp, F.4, Author           
Schulz, E.-C.1, 2, Author           
Affiliations:
1Hamburg Centre for Ultrafast Imaging, Universität Hamburg, HARBOR, ou_persistent22              
2Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
3European Molecular Biology Laboratory (EMBL), Hamburg Unit c/o Deutsches Elektronen Synchrotron (DESY), ou_persistent22              
4Machine Physics, Scientific Service Units, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_2074322              

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 Abstract: We present a new environmental enclosure for fixed-target, serial crystallography enabling full control of both the temperature and humidity. While maintaining the relative humidity to within a percent, this enclosure provides access to X-ray diffraction experiments in a wide temperature range from below 10 °C to above 80 °C. Coupled with the LAMA method, time-resolved serial crystallography experiments can now be carried out at truly physiological temperatures, providing fundamentally new insight into protein function. Using the hyperthermophile enzyme xylose isomerase, we demonstrate changes in the electron density as a function of increasing temperature and time. This method provides the necessary tools to successfully carry out multi-dimensional serial crystallography.

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Language(s): eng - English
 Dates: 2021-11-08
 Publication Status: Published online
 Pages: 21
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 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: 10.1101/2021.11.07.467596
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