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  Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities

Mertes, N., Busch, M., Huppertz, M.-C., Hacker, C. N., Gürth, C.-M., Kühn, S., et al. (2021). Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities. bioRxiv, 1-16. doi:10.1101/2021.11.13.468356.

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 Creators:
Mertes, Nicole1, Author              
Busch, Marvin1, Author              
Huppertz, Magnus-Carsten1, Author              
Hacker, Christina Nicole1, Author              
Gürth, Clara-Marie2, Author              
Kühn, Stefanie1, Author              
Hiblot, Julien1, Author              
Koch, Birgit1, Author              
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              
2Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Fluorescence, Bioluminescence, Calcium Indicator
 Abstract: We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The indicators can be specifically localized to different cellular compartments and are compatible with both fluorescence and bioluminescence readouts through conjugation to HaloTag fusion proteins. Importantly, their increase in fluorescence upon localization enables no-wash live-cell imaging, which greatly facilitates their use in biological assays. Applications as fluorescent indicators in rat hippocampal neurons include the detection of single action potentials and of calcium fluxes in the endoplasmic reticulum (ER). Applications as bioluminescent indicators include the recording of the pharmacological modulation of nuclear calcium in high-throughput-compatible assays. The versatility and remarkable ease of use of these indicators make them powerful tools for bioimaging and bioassays.

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Language(s): eng - English
 Dates: 2021-11-14
 Publication Status: Published online
 Pages: 16
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Title: bioRxiv
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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 16 Identifier: -