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  Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities

Mertes, N., Busch, M., Huppertz, M.-C., Hacker, C. N., Gürth, C.-M., Kühn, S., et al. (2022). Fluorescent and bioluminescent calcium indicators with tuneable colors and affinities. Journal of the American Chemical Society, 144(15), 6928-6935. doi:10.1021/jacs.2c01465.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-A174-E Version Permalink: https://hdl.handle.net/21.11116/0000-000D-4F74-9
Genre: Journal Article

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 Creators:
Mertes, Nicole1, Author           
Busch, Marvin1, Author           
Huppertz, Magnus-Carsten1, Author           
Hacker, Christina Nicole1, Author           
Gürth, Clara-Marie2, Author           
Kühn, Stefanie1, Author           
Hiblot, Julien1, Author           
Koch, Birgit1, Author           
Johnsson, Kai1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              
2Optical Nanoscopy, Max Planck Institute for Medical Research, Max Planck Society, ou_2364730              

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Free keywords: Fluorescence, Bioluminescence, Calcium Indicator
 Abstract: We introduce a family of bright, rhodamine-based calcium indicators with tuneable affinities and colors. The indicators can be specifically localized to different cellular compartments and are compatible with both fluorescence and bioluminescence readouts through conjugation to HaloTag fusion proteins. Importantly, their increase in fluorescence upon localization enables no-wash live-cell imaging, which greatly facilitates their use in biological assays. Applications as fluorescent indicators in rat hippocampal neurons include the detection of single action potentials and of calcium fluxes in the endoplasmic reticulum (ER). Applications as bioluminescent indicators include the recording of the pharmacological modulation of nuclear calcium in high-throughput-compatible assays. The versatility and remarkable ease of use of these indicators make them powerful tools for bioimaging and bioassays.

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Language(s): eng - English
 Dates: 2022-02-072022-04-052022-04-20
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacs.2c01465
URI: https://pubmed.ncbi.nlm.nih.gov/35380808/
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Title: Journal of the American Chemical Society
  Other : JACS
  Abbreviation : J. Am. Chem. Soc.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 144 (15) Sequence Number: - Start / End Page: 6928 - 6935 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870