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  Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs

Kemter, E., Müller, A., Neukam, M., Ivanova, A., Klymiuk, N., Renner, S., et al. (2021). Sequential in vivo labeling of insulin secretory granule pools in INS-SNAP transgenic pigs. Proceedings of the National Academy of Sciences of the United States of America, 118(37): e2107665118, pp. 1-8. doi:10.1073/pnas.2107665118.

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 Creators:
Kemter, Elisabeth, Author
Müller, Andreas, Author
Neukam, Martin, Author
Ivanova, Anna, Author
Klymiuk, Nikolai, Author
Renner, Simone, Author
Yang, Kaiyuan, Author
Broichhagen, Johannes1, Author              
Kurome, Mayuko, Author
Zakhartchenko, Valeri, Author
Kessler, Barbara, Author
Knoch, Klaus-Peter, Author
Bickle, Marc, Author
Ludwig, Barbara, Author
Johnsson, Kai1, Author              
Lickert, Heiko, Author
Kurth, Thomas, Author
Wolf, Eckhard, Author
Solimena, Michele, Author
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: diabetes mellitus; insulin turnover; pig model; β cell.
 Abstract: β cells produce, store, and secrete insulin upon elevated blood glucose levels. Insulin secretion is a highly regulated process. The probability for insulin secretory granules to undergo fusion with the plasma membrane or being degraded is correlated with their age. However, the molecular features and stimuli connected to this behavior have not yet been fully understood. Furthermore, our understanding of β cell function is mostly derived from studies of ex vivo isolated islets in rodent models. To overcome this translational gap and study insulin secretory granule turnover in vivo, we have generated a transgenic pig model with the SNAP-tag fused to insulin. We demonstrate the correct targeting and processing of the tagged insulin and normal glycemic control of the pig model. Furthermore, we show specific single- and dual-color granular labeling of in vivo-labeled pig pancreas. This model may provide unprecedented insights into the in vivo insulin secretory granule behavior in an animal close to humans.

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Language(s): eng - English
 Dates: 2021-04-262021-09-14
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 118 (37) Sequence Number: e2107665118 Start / End Page: 1 - 8 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230