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  Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators

Pennemann, F. L., Mussabekova, A., Urban, C., Stukalov, A., Andersen, L. L., Grass, V., et al. (2021). Cross-species analysis of viral nucleic acid interacting proteins identifies TAOKs as innate immune regulators. Nature Communications, 12(1): 7009. doi:10.1038/s41467-021-27192-w.

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 Creators:
Pennemann, Friederike L.1, Author
Mussabekova, Assel1, Author
Urban, Christian1, Author
Stukalov, Alexey1, Author
Andersen, Line Lykke1, Author
Grass, Vincent1, Author
Lavacca, Teresa Maria1, Author
Holze, Cathleen2, Author              
Oubraham, Lila1, Author
Benamrouche, Yasmine1, Author
Girardi, Enrico1, Author
Boulos, Rasha E.1, Author
Hartmann, Rune1, Author
Superti-Furga, Giulio1, Author
Habjan, Matthias2, Author              
Imler, Jean-Luc1, Author
Meignin, Carine1, Author
Pichlmair, Andreas2, Author              
Affiliations:
1external, ou_persistent22              
2Pichlmair, Andreas / Innate Immunity, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565166              

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Free keywords: DOUBLE-STRANDED-RNA; LONG NONCODING RNA; SYSTEMATIC IDENTIFICATION; COMPUTATIONAL PLATFORM; COMPREHENSIVE ANALYSIS; 5'-TRIPHOSPHATE RNA; ANTIVIRAL RESPONSE; KAPPA-B; RIG-I; RECOGNITIONScience & Technology - Other Topics;
 Abstract: The cell intrinsic antiviral response of multicellular organisms developed over millions of years and critically relies on the ability to sense and eliminate viral nucleic acids. Here we use an affinity proteomics approach in evolutionary distant species (human, mouse and fly) to identify proteins that are conserved in their ability to associate with diverse viral nucleic acids. This approach shows a core of orthologous proteins targeting viral genetic material and species-specific interactions. Functional characterization of the influence of 181 candidates on replication of 6 distinct viruses in human cells and flies identifies 128 nucleic acid binding proteins with an impact on virus growth. We identify the family of TAO kinases (TAOK1, -2 and -3) as dsRNA-interacting antiviral proteins and show their requirement for type-I interferon induction. Depletion of TAO kinases in mammals or flies leads to an impaired response to virus infection characterized by a reduced induction of interferon stimulated genes in mammals and impaired expression of srg1 and diedel in flies. Overall, our study shows a larger set of proteins able to mediate the interaction between viral genetic material and host factors than anticipated so far, attesting to the ancestral roots of innate immunity and to the lineage-specific pressures exerted by viruses. Whether there are conserved nucleic acid (NA) binding proteins across species is not fully known. Using data from human, mouse and fly, the authors identify common binders, implicate TAOKs and show that these kinases bind NAs across species and promote virus defence in mammalian cells.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Published online
 Pages: 22
 Publishing info: -
 Table of Contents: We further thank Korbinian Mayr, Igor Paron, and Gaby Sowa for maintaining mass spectrometers and the MPI-B core facility, especially Judith Scholz, Leopold Urich, Sabine Suppmann, and Stephan Uebel, for support...
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 12 (1) Sequence Number: 7009 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723