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  Photocaged hoechst enables subnuclear visualization and cell selective staining of DNA in vivo

Lämmle, C. A., Varady, A., Müller, T. G., Sturtzel, C., Riepl, M., Mathes, B., et al. (2021). Photocaged hoechst enables subnuclear visualization and cell selective staining of DNA in vivo. ChemBioChem: A European Journal of Chemical Biology, 22(3), 548-556. doi:10.1002/cbic.202000465.

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 Creators:
Lämmle, Carina A.1, Author           
Varady, Adam, Author
Müller, Thorsten G, Author
Sturtzel, Caterina, Author
Riepl, Michael, Author
Mathes, Bettina, Author
Eichhorst, Jenny, Author
Sporbert, Anje, Author
Lehmann, Martin, Author
Kräusslich, Hans-Georg, Author
Distel, Martin, Author
Broichhagen, Johannes1, Author           
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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Free keywords: DNA; Hoechst; labeling; microscopy; zebrafish
 Abstract: Selective targeting of DNA by means of fluorescent labeling has become a mainstay in the life sciences. While genetic engineering serves as a powerful technique and allows the visualization of nucleic acid by using DNA-targeting fluorescent fusion proteins in a cell-type- and subcellular-specific manner, it relies on the introduction of foreign genes. On the other hand, DNA-binding small fluorescent molecules can be used without genetic engineering, but they are not spatially restricted. Herein, we report a photocaged version of the DNA dye Hoechst33342 (pcHoechst), which can be uncaged by using UV to blue light for the selective staining of chromosomal DNA in subnuclear regions of live cells. Expanding its application to a vertebrate model organism, we demonstrate uncaging in epithelial cells and short-term cell tracking in vivo in zebrafish. We envision pcHoechst as a valuable tool for targeting and interrogating DNA with precise spatiotemporal resolution in living cells and wild-type organisms.

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Language(s): eng - English
 Dates: 2020-09-172020-07-132020-09-252020-11-022021-02-02
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: ChemBioChem: A European Journal of Chemical Biology
  Other : ChemBioChem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : Wiley-VCH
Pages: - Volume / Issue: 22 (3) Sequence Number: - Start / End Page: 548 - 556 Identifier: ISSN: 1439-4227
CoNE: https://pure.mpg.de/cone/journals/resource/110978984568897_1