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  Interphase epichromatin: last refuge for the 30-nm chromatin fiber?

Xu, P., Mahamid, J., Dombrowski, M., Baumeister, W., Olins, A. L., & Olins, D. E. (2021). Interphase epichromatin: last refuge for the 30-nm chromatin fiber? Chromosoma, 130(2-3), 91-102. doi:10.1007/s00412-021-00759-8.

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https://rdcu.be/cEK94 (Publisher version)
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 Creators:
Xu, Peng1, Author
Mahamid, Julia1, Author              
Dombrowski, Marco1, Author
Baumeister, Wolfgang1, Author              
Olins, Ada L.2, Author
Olins, Donald E.2, Author
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
2external, ou_persistent22              

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Free keywords: ELECTRON-MICROSCOPE OBSERVATIONS; LAMIN B RECEPTOR; ENVELOPE-LIMITED MONOLAYERS; STRUCTURAL UNITS; IN-SITU; ORGANIZATION; NUCLEOSOME; CHROMOSOMES; THREADS; SHEETSBiochemistry & Molecular Biology; Genetics & Heredity; Nucleosomes; Epichromatin; Cryo-electron tomography; Envelope-limited chromatin sheets;
 Abstract: "Interphase epichromatin" describes the surface of chromatin located adjacent to the interphase nuclear envelope. It was discovered in 2011 using a bivalent anti-nucleosome antibody (mAb PL2-6), now known to be directed against the nucleosome acidic patch. The molecular structure of interphase epichromatin is unknown, but is thought to be heterochromatic with a high density of "exposed" acidic patches. In the 1960s, transmission electron microscopy of fixed, dehydrated, sectioned, and stained inactive chromatin revealed "unit threads," frequently organized into parallel arrays at the nuclear envelope, which were interpreted as regular helices with similar to 30-nm center-to-center distance. Also observed in certain cell types, the nuclear envelope forms a "sandwich" around a layer of closely packed unit threads (ELCS, envelope-limited chromatin sheets). Discovery of the nucleosome in 1974 led to revised helical models of chromatin. But these models became very controversial and the existence of in situ 30-nm chromatin fibers has been challenged. Development of cryo-electron microscopy (Cryo-EM) gave hope that in situ chromatin fibers, devoid of artifacts, could be structurally defined. Combining a contrast-enhancing phase plate and cryo-electron tomography (Cryo-ET), it is now possible to visualize chromatin in a "close-to-native" situation. ELCS are particularly interesting to study by Cryo-ET. The chromatin sheet appears to have two layers of similar to 30-nm chromatin fibers arranged in a criss-crossed pattern. The chromatin in ELCS is continuous with adjacent interphase epichromatin. It appears that hydrated similar to 30-nm chromatin fibers are quite rare in most cells, possibly confined to interphase epichromatin at the nuclear envelope.

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Language(s): eng - English
 Dates: 2021-09
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

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Title: Chromosoma
  Other : Chromosoma
Source Genre: Journal
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Publ. Info: Wien : Springer-Verlag
Pages: - Volume / Issue: 130 (2-3) Sequence Number: - Start / End Page: 91 - 102 Identifier: ISSN: 0009-5915
CoNE: https://pure.mpg.de/cone/journals/resource/991042749609288