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  Neurons differentiate magnitude and location of mechanical stimuli

Gaub, B. M., Kasuba, K. C., Macé, E., Strittmatter, T., Laskowski, P. R., Geissler, S. A., et al. (2020). Neurons differentiate magnitude and location of mechanical stimuli. Proceedings of the National Academy of Sciences of the United States of America, 117(2), 848-856. doi:10.1073/pnas.1909933117.

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 Creators:
Gaub, B. M., Author
Kasuba, K. C., Author
Macé, Emilie1, Author              
Strittmatter, T., Author
Laskowski, P. R., Author
Geissler, S. A., Author
Hierlemann, A., Author
Fussenegger, M., Author
Roska, B., Author
Muller, D. J., Author
Affiliations:
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland, ou_persistent22              

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Free keywords: atomic force microscopy calcium response mechanobiology mechanosensitivity cortex neurons calcium-channels cation channel brain trpv1 threshold stretch piezo1 form Science & Technology - Other Topics
 Abstract: Neuronal activity can be modulated by mechanical stimuli. To study this phenomenon quantitatively, we mechanically stimulated rat cortical neurons by shear stress and local indentation. Neurons show 2 distinct responses, classified as transient and sustained. Transient responses display fast kinetics, similar to spontaneous neuronal activity, whereas sustained responses last several minutes before returning to baseline. Local soma stimulations with micrometersized beads evoke transient responses at low forces of similar to 220 nN and pressures of similar to 5.6 kPa and sustained responses at higher forces of similar to 360 nN and pressures of similar to 9.2 kPa. Among the neuronal compartments, axons are highly susceptible to mechanical stimulation and predominantly show sustained responses, whereas the less susceptible dendrites predominantly respond transiently. Chemical perturbation experiments suggest that mechanically evoked responses require the influx of extracellular calcium through ion channels. We propose that subtraumatic forces/pressures applied to neurons evoke neuronal responses via nonspecific gating of ion channels.

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Language(s): eng - English
 Dates: 2020-01-14
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000508976200014
DOI: 10.1073/pnas.1909933117
ISSN: 0027-8424
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Affiliations:
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 117 (2) Sequence Number: - Start / End Page: 848 - 856 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230