English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Synchrotron-based nano-X-ray absorption near-edge structure revealing intracellular heterogeneity of iron species in magnetotactic bacteria

Chevrier, D. M., Cerdá Doñate, E., Park, Y., Cacho-Nerin, F., Gomez-Gonzalez, M., Uebe, R., et al. (2022). Synchrotron-based nano-X-ray absorption near-edge structure revealing intracellular heterogeneity of iron species in magnetotactic bacteria. Small science, 2(3): 2100089. doi:10.1002/smsc.202100089.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Article.pdf (Publisher version), 3MB
Name:
Article.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-

Locators

show

Creators

show
hide
 Creators:
Chevrier, Daniel M.1, Author
Cerdá Doñate, Elisa1, Author              
Park, Yeseul, Author
Cacho-Nerin, Fernando, Author
Gomez-Gonzalez, Miguel, Author
Uebe, René, Author
Faivre, Damien1, Author              
Affiliations:
1Damien Faivre, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863290              

Content

show
hide
Free keywords: biomineralization, magnetite, magnetosomes, magnetotactic bacteria, nano-X-ray absorption near-edge structure, single cells, X-ray microscopy
 Abstract: Magnetotactic bacteria (MTB) sequester iron from the environment to biomineralize magnetite or greigite nanoparticles in magnetosome organelles, though the necessity of intracellular iron storage for the formation process is still in question. Understanding the role of iron storage would make clear the contribution of MTB in geochemical iron cycling and its potential importance during the biosynthesis of application-relevant magnetic nanoparticles. Herein, how scanning X-ray fluorescence microscopy (SXFM) and nanoscale X-ray absorption near-edge structure (nano-XANES) mapping can spatially and chemically identify intracellular iron species is reported, creating an opportunity to examine the role of iron storage in magnetite biomineralization at the single-cell level. Fe K-edge nano-XANES measurements of Magnetospirillum gryphiswaldense in varied iron media conditions and iron storage capacity reveal a significant quantity of intracellular iron heterogeneities through a distinction between formed magnetosomes and intracellular iron material. This intracellular iron component is found in both early and late stages of biomineralization. The capabilities of nano-XANES in providing an experimental advantage in the multidisciplinary field of biomineralization are highlighted.

Details

show
hide
Language(s): eng - English
 Dates: 2021-12-232022
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1002/smsc.202100089
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Small science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 2 (3) Sequence Number: 2100089 Start / End Page: - Identifier: ISSN: 2688-4046