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  Synthetic plant glycan microarrays as tools for plant biology

Ruprecht, C., & Pfrengle, F. (2022). Synthetic plant glycan microarrays as tools for plant biology. In J. M. Walker (Ed.), Methods in molcecular biology (pp. 115-125). New York, NY: Springer US. doi:10.1007/978-1-0716-2148-6_7.

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Article.pdf (Publisher version), 490KB
 
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 Creators:
Ruprecht, Colin1, Author              
Pfrengle, Fabian1, Author              
Affiliations:
1Fabian Pfrengle, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863303              

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Free keywords: Glycan microarray; Plant cell wall oligosaccharides; Cell wall glycan-directed antibodies; Glycan-binding proteins; Lectins
 Abstract: Chemically synthesized plant oligosaccharides have recently evolved as powerful molecular tools for plant cell wall biology. Synthetic plant glycan microarrays equipped with these oligosaccharides enable high-throughput analyses of glycan-binding proteins and carbohydrate-active enzymes. To produce these glycan microarrays, small amounts of glycan solution are printed on suitable surfaces for covalent or non-covalent immobilization. Synthetic plant glycan microarrays have been used for example to map the epitopes of plant cell wall-directed antibodies, to characterize glycosyl hydrolases and glycosyl transferases, and to analyze lectin binding. In this chapter, detailed experimental procedures for the production of synthetic glycan microarrays and their use for the characterization of cell wall glycan-directed antibodies are described.

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Language(s): eng - English
 Dates: 2022-01-012022
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-2148-6_7
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Title: Methods in molcecular biology
Source Genre: Series
 Creator(s):
Walker, John M., Editor
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Publ. Info: New York, NY : Springer US
Pages: - Volume / Issue: 2460 Sequence Number: - Start / End Page: 115 - 125 Identifier: ISSN: 1940-6029

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Title: Glycan microarrays : methods and protocols
Source Genre: Book
 Creator(s):
Kilcoyne, Michelle, Editor
Gerlach, Jared Q., Editor
Affiliations:
-
Publ. Info: New York : Springer US
Pages: XI, 253 Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISBN: 978-1-0716-2148-6