English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Finding the best clearing approach: Towards 3D wide-scale multimodal imaging of aged human brain tissue

Rusch, H., Brammerloh, M., Stieler, J., Sonntag, M., Mohammadi, S., Weiskopf, N., et al. (2022). Finding the best clearing approach: Towards 3D wide-scale multimodal imaging of aged human brain tissue. NeuroImage, 247: 118832. doi:10.1016/j.neuroimage.2021.118832.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Rusch_2022.pdf (Publisher version), 4MB
Name:
Rusch_2022.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Rusch, Henriette1, Author
Brammerloh, Malte2, 3, 4, Author              
Stieler, Jens1, Author
Sonntag, Mandy1, Author
Mohammadi, Siawoosh2, 5, Author              
Weiskopf, Nikolaus2, 3, Author              
Arendt, Thomas1, Author
Kirilina, Evgeniya2, 6, Author              
Morawski, Markus1, 2, Author              
Affiliations:
1Faculty of Medicine, Paul Flechsig Institute for Brain Research, University of Leipzig, Germany, ou_persistent22              
2Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              
3Felix Bloch Institute for Solid State Physics, University of Leipzig, Germany, ou_persistent22              
4International Max Planck Research School on Neuroscience of Communication, Leipzig, Germany, ou_persistent22              
5Department of Systems Neuroscience, University Medical Center Hamburg-Eppendorf, Germany, ou_persistent22              
6Center for Cognitive Neuroscience Berlin (CCNB), FU Berlin, Germany, ou_persistent22              

Content

show
hide
Free keywords: CLARITY; IDISCO; Immunohistochemistry; Light sheet microscopy; Tissue clearing
 Abstract: The accessibility of new wide-scale multimodal imaging techniques led to numerous clearing techniques emerging over the last decade. However, clearing mesoscopic-sized blocks of aged human brain tissue remains an extremely challenging task. Homogenizing refractive indices and reducing light absorption and scattering are the foundation of tissue clearing. Due to its dense and highly myelinated nature, especially in white matter, the human brain poses particular challenges to clearing techniques. Here, we present a comparative study of seven tissue clearing approaches and their impact on aged human brain tissue blocks (> 5 mm). The goal was to identify the most practical and efficient method in regards to macroscopic transparency, brief clearing time, compatibility with immunohistochemical processing and wide-scale multimodal microscopic imaging. We successfully cleared 26 × 26 × 5 mm3-sized human brain samples with two hydrophilic and two hydrophobic clearing techniques. Optical properties as well as light and antibody penetration depths highly vary between these methods. In addition to finding the best clearing approach, we compared three microscopic imaging setups (the Zeiss Laser Scanning Microscope (LSM) 880 , the Miltenyi Biotec Ultramicroscope ll (UM ll) and the 3i Marianas LightSheet microscope) regarding optimal imaging of large-scale tissue samples. We demonstrate that combining the CLARITY technique (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging compatible Tissue hYdrogel) with the Zeiss LSM 880 and combining the iDISCO technique (immunolabeling-enabled three-dimensional imaging of solvent-cleared organs) with the Miltenyi Biotec UM ll are the most practical and efficient approaches to sufficiently clear aged human brain tissue and generate 3D microscopic images. Our results point out challenges that arise from seven clearing and three imaging techniques applied to non-standardized tissue samples such as aged human brain tissue.

Details

show
hide
Language(s): eng - English
 Dates: 2021-12-132021-06-142021-12-162021-12-172022-02-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.neuroimage.2021.118832
Other: epub 2021
PMID: 34929383
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: NeuroImage
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Orlando, FL : Academic Press
Pages: - Volume / Issue: 247 Sequence Number: 118832 Start / End Page: - Identifier: ISSN: 1053-8119
CoNE: https://pure.mpg.de/cone/journals/resource/954922650166