English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Distal and proximal cis-regulatory elements sense X chromosome dosage and developmental state at the Xist locus

Gjaltema, R., Schwämmle, T., Kautz, P., Robson, M., Schöpflin, R., Lustig, L. R., et al. (2022). Distal and proximal cis-regulatory elements sense X chromosome dosage and developmental state at the Xist locus. Molecular Cell, 82, 1-19. doi:10.1016/j.molcel.2021.11.023.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
Gjaltema et al_2021.pdf (Publisher version), 5MB
Name:
Gjaltema et al_2021.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2021 The Author(s)

Locators

show

Creators

show
hide
 Creators:
Gjaltema, Rutger1, Author              
Schwämmle, Till1, Author              
Kautz, Pauline1, Author              
Robson, Michael, Author
Schöpflin, Robert, Author
Lustig, Liat Ravid1, Author              
Brandenburg, Lennart1, Author
Dunkel, Ilona1, Author              
Vechiatto, Carolina1, Author              
Ntini, Evgenia1, Author
Mutzel, Verena1, Author              
Schmiedel, Vera1, Author
Marsico, Annalisa, Author
Mundlos, Stefan2, Author              
Schulz, Edda G.1, Author              
Affiliations:
1Systems Epigenetics (Edda G. Schulz), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117286              
2Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

Content

show
hide
Free keywords: X-chromosome inactivation Xist CRISPR screens enhancers lncRNA Xert chromatin modifications epigenetics CRISPRi
 Abstract: Developmental genes such as Xist, which initiates X chromosome inactivation, are controlled by complex cis-regulatory landscapes, which decode multiple signals to establish specific spatiotemporal expression patterns. Xist integrates information on X chromosome dosage and developmental stage to trigger X inactivation in the epiblast specifically in female embryos. Through a pooled CRISPR screen in differentiating mouse embryonic stem cells, we identify functional enhancer elements of Xist at the onset of random X inactivation. Chromatin profiling reveals that X-dosage controls the promoter-proximal region, while differentiation cues activate several distal enhancers. The strongest distal element lies in an enhancer cluster associated with a previously unannotated Xist-enhancing regulatory transcript, which we named Xert. Developmental cues and X-dosage are thus decoded by distinct regulatory regions, which cooperate to ensure female-specific Xist upregulation at the correct developmental time. With this study, we start to disentangle how multiple, functionally distinct regulatory elements interact to generate complex expression patterns in mammals.

Details

show
hide
Language(s): eng - English
 Dates: 2021-11-232021-12-202022-01-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.molcel.2021.11.023
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Molecular Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 82 Sequence Number: - Start / End Page: 1 - 19 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929