English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Tuning epithelial cell–cell adhesion and collective dynamics with functional DNA-E-Cadherin hybrid linkers

Schoenit, A., Lo Giudice, C., Hahnen, N., Ollech, D., Jahnke, K., Göpfrich, K., et al. (2022). Tuning epithelial cell–cell adhesion and collective dynamics with functional DNA-E-Cadherin hybrid linkers. Nano Letters, 22(1), 302-310. doi:10.1021/acs.nanolett.1c03780.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/21.11116/0000-0009-BCFA-A Version Permalink: https://hdl.handle.net/21.11116/0000-0009-DF0A-2
Genre: Journal Article

Files

show Files
hide Files
:
NanoLett_22_2022_302.pdf (Any fulltext), 9MB
 
File Permalink:
-
Name:
NanoLett_22_2022_302.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NanoLett_22_2022_302_Suppl1.pdf (Supplementary material), 2MB
 
File Permalink:
-
Name:
NanoLett_22_2022_302_Suppl1.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NanoLett_22_2022_302_Suppl2.avi (Supplementary material), 8MB
 
File Permalink:
-
Name:
NanoLett_22_2022_302_Suppl2.avi
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NanoLett_22_2022_302_Suppl3.avi (Supplementary material), 109MB
 
File Permalink:
-
Name:
NanoLett_22_2022_302_Suppl3.avi
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NanoLett_22_2022_302_Suppl4.avi (Supplementary material), 16MB
 
File Permalink:
-
Name:
NanoLett_22_2022_302_Suppl4.avi
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
NanoLett_22_2022_302_Suppl5.avi (Supplementary material), 57MB
 
File Permalink:
-
Name:
NanoLett_22_2022_302_Suppl5.avi
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
https://pubs.acs.org/doi/pdf/10.1021/acs.nanolett.1c03780 (Any fulltext)
Description:
-
OA-Status:
Locator:
https://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.1c03780/suppl_file/nl1c03780_si_001.pdf (Supplementary material)
Description:
-
OA-Status:
Locator:
https://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.1c03780/suppl_file/nl1c03780_si_002.avi (Supplementary material)
Description:
-
OA-Status:
Locator:
https://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.1c03780/suppl_file/nl1c03780_si_003.avi (Supplementary material)
Description:
-
OA-Status:
Locator:
https://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.1c03780/suppl_file/nl1c03780_si_004.avi (Supplementary material)
Description:
-
OA-Status:
Locator:
https://pubs.acs.org/doi/suppl/10.1021/acs.nanolett.1c03780/suppl_file/nl1c03780_si_005.avi (Supplementary material)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Schoenit, Andreas1, Author           
Lo Giudice, Cristina1, Author           
Hahnen, Nina1, Author           
Ollech, Dirk1, Author           
Jahnke, Kevin1, Author           
Göpfrich, Kerstin1, Author           
Cavalcanti-Adam, Elisabetta Ada1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: cell−cell adhesion strength, E-cadherin, DNA nanotechnology, adherens junction, epithelial cells, collective migration, DNA−protein hybrid, mechanotransduction
 Abstract: The binding strength between epithelial cells is crucial for tissue integrity, signal transduction and collective cell dynamics. However, there is no experimental approach to precisely modulate cell–cell adhesion strength at the cellular and molecular level. Here, we establish DNA nanotechnology as a tool to control cell–cell adhesion of epithelial cells. We designed a DNA-E-cadherin hybrid system consisting of complementary DNA strands covalently bound to a truncated E-cadherin with a modified extracellular domain. DNA sequence design allows to tune the DNA-E-cadherin hybrid molecular binding strength, while retaining its cytosolic interactions and downstream signaling capabilities. The DNA-E-cadherin hybrid facilitates strong and reversible cell–cell adhesion in E-cadherin deficient cells by forming mechanotransducive adherens junctions. We assess the direct influence of cell–cell adhesion strength on intracellular signaling and collective cell dynamics. This highlights the scope of DNA nanotechnology as a precision technology to study and engineer cell collectives.

Details

show
hide
Language(s): eng - English
 Dates: 2021-12-122021-09-302021-12-232022-01-12
 Publication Status: Issued
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/acs.nanolett.1c03780
URI: https://pubmed.ncbi.nlm.nih.gov/34939414/
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 22 (1) Sequence Number: - Start / End Page: 302 - 310 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403