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  Tuning epithelial cell–cell adhesion and collective dynamics with functional DNA-E-Cadherin hybrid linkers

Schoenit, A., Lo Giudice, C., Hahnen, N., Ollech, D., Jahnke, K., Göpfrich, K., et al. (2022). Tuning epithelial cell–cell adhesion and collective dynamics with functional DNA-E-Cadherin hybrid linkers. Nano Letters, 22(1), 302-310. doi:10.1021/acs.nanolett.1c03780.

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 Creators:
Schoenit, Andreas1, Author              
Lo Giudice, Cristina1, Author              
Hahnen, Nina1, Author              
Ollech, Dirk1, Author              
Jahnke, Kevin1, Author              
Göpfrich, Kerstin1, Author              
Cavalcanti-Adam, Elisabetta Ada1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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Free keywords: cell−cell adhesion strength, E-cadherin, DNA nanotechnology, adherens junction, epithelial cells, collective migration, DNA−protein hybrid, mechanotransduction
 Abstract: The binding strength between epithelial cells is crucial for tissue integrity, signal transduction and collective cell dynamics. However, there is no experimental approach to precisely modulate cell–cell adhesion strength at the cellular and molecular level. Here, we establish DNA nanotechnology as a tool to control cell–cell adhesion of epithelial cells. We designed a DNA-E-cadherin hybrid system consisting of complementary DNA strands covalently bound to a truncated E-cadherin with a modified extracellular domain. DNA sequence design allows to tune the DNA-E-cadherin hybrid molecular binding strength, while retaining its cytosolic interactions and downstream signaling capabilities. The DNA-E-cadherin hybrid facilitates strong and reversible cell–cell adhesion in E-cadherin deficient cells by forming mechanotransducive adherens junctions. We assess the direct influence of cell–cell adhesion strength on intracellular signaling and collective cell dynamics. This highlights the scope of DNA nanotechnology as a precision technology to study and engineer cell collectives.

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Language(s): eng - English
 Dates: 2021-12-122021-09-302021-12-232022-01-12
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Nano Letters
  Abbreviation : Nano Lett.
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 22 (1) Sequence Number: - Start / End Page: 302 - 310 Identifier: ISSN: 1530-6984
CoNE: https://pure.mpg.de/cone/journals/resource/110978984570403