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  Similarities and differences in the way transmembrane-type ligands interact with the Elk subclass of Eph receptors

Brambilla, R., Brückner, K., Orioli, D., Bergemann, A. D., Flanagan, J. G., & Klein, R. (1996). Similarities and differences in the way transmembrane-type ligands interact with the Elk subclass of Eph receptors. Molecular and Cellular Neuroscience, 8(2-3), 199-209. doi:10.1006/mcne.1996.0057.

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Brambilla, R., Author
Brückner, K., Author
Orioli, D., Author
Bergemann, A. D., Author
Flanagan, J. G., Author
Klein, Rüdiger1, Author           
Affiliations:
1European Molecular Biology Laboratory, Heidelberg, ou_persistent22              

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Free keywords: protein-tyrosine kinase neurotrophic factor nervous-system family cloning expression identification member elf-1 mek4 Neurosciences & Neurology
 Abstract: The Eph family of receptor tyrosine kinases and their cell surface bound ligands have been implicated in a number of developmental processes, including axon pathfinding and fasciculation, as well as patterning in the central nervous system. To better understand the complex signaling events taking place, we have undertaken a comparative analysis of ligand-receptor interactions between a subset of ligands, those that are tethered to the cell surface via a transmembrane domain, and a subset of Eph receptors, the so-called Elk subclass. Based on binding characteristics, receptor autophosphorylation, and cellular transformation assays, we find that the transmembrane-type ligands Lerk2 and Elf2 have common and specific receptors within the Elk subclass of receptors. The common receptors Cek10 and Elk bind and signal in response to Lerk2 and Elf2, whereas the Myk1 receptor is specific for Elf2. Elf2, however, fails to signal through Cek5 in a cellular transformation assay, suggesting that Lerk2 may be the preferred Cek5 ligand in vivo. A recently identified third transmembrane-type ligand, Elf3, specifically, but weakly, binds Cek10 and only induces focus formation when activated by C-terminal truncation. This suggests that the physiological Elf3 receptor may have yet to be identified. Knowledge regarding functional ligand-receptor interactions as presented in this study will be important for the design and interpretation of in vivo experiments, e.g., loss-of-function studies in transgenic mice.

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Language(s): eng - English
 Dates: 1996
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:A1996VT00900013
DOI: 10.1006/mcne.1996.0057
ISSN: 1044-7431
 Degree: -

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Title: Molecular and Cellular Neuroscience
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 8 (2-3) Sequence Number: - Start / End Page: 199 - 209 Identifier: ISSN: 1044-7431
CoNE: https://pure.mpg.de/cone/journals/resource/954922650153