English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Kindlin-3 maintains marginal zone B cells but confines follicular B cell activation and differentiation

Harzschel, A., Li, L., Krenn, P., Szenes-Nagy, E., Andrieux, G., Bayer, E., et al. (2021). Kindlin-3 maintains marginal zone B cells but confines follicular B cell activation and differentiation. Journal of Leukocyte Biology, 1-14. doi:10.1002/JLB.1HI0621-313R.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
J Leukocyte Bio - 2021 - H rzschel - Kindlin‐3 maintains marginal zone B cells but confines follicular B cell activation.pdf (Publisher version), 11MB
Name:
J Leukocyte Bio - 2021 - H rzschel - Kindlin‐3 maintains marginal zone B cells but confines follicular B cell activation.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2021 The Authors.
:
jlb11055-sup-0001-suppmat.pdf (Supplementary material), 2MB
Name:
jlb11055-sup-0001-suppmat.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Harzschel, Andrea1, Author
Li, Lixia1, Author
Krenn, Peter2, Author              
Szenes-Nagy, Eva1, Author
Andrieux, Geoffroy1, Author
Bayer, Elisabeth1, Author
Pfeifer, Dietmar1, Author
Polcik, Laura1, Author
Denk, Ursula1, Author
Hopner, Jan P.1, Author
Karabatak, Elif1, Author
Danner, Danielle-Justine1, Author
Tangermann, Simone1, Author
Kenner, Lukas1, Author
Jumaa, Hassan1, Author
Greil, Richard1, Author
Borries, Melanie1, Author
Ruppert, Raphael2, Author              
Maity, Palash C.1, Author
Hartmann, Tanja Nicole1, Author
Affiliations:
1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              

Content

show
hide
Free keywords: LYMPHOCYTIC-LEUKEMIA CELLS; INTEGRIN ACTIVATION; BONE-MARROW; HEMATOPOIETIC PROGENITORS; LYMPH-NODES; ADHESION; RECEPTOR; CXCR4; VLA-4; LOCALIZATIONCell Biology; Hematology; Immunology; B cell activation; CXCR5; follicular B cells; Integrin adhesion; marginal zone B cells;
 Abstract: Integrin-mediated interactions between hematopoietic cells and their microenvironment are important for the development and function of immune cells. Here, the role of the integrin adaptor Kindlin-3 in B cell homeostasis is studied. Comparing the individual steps of B cell development in B cell-specific Kindlin-3 or alpha4 integrin knockout mice, we found in both conditions a phenotype of reduced late immature, mature, and recirculating B cells in the bone marrow. In the spleen, constitutive B cell-specific Kindlin-3 knockout caused a loss of marginal zone B cells and an unexpected expansion of follicular B cells. Alpha4 integrin deficiency did not induce this phenotype. In Kindlin-3 knockout B cells VLA-4 as well as LFA-1-mediated adhesion was abrogated, and short-term homing of these cells in vivo was redirected to the spleen. Upon inducible Kindlin-3 knockout, marginal zone B cells were lost due to defective retention within 2 weeks, while follicular B cell numbers were unaltered. Kindlin-3 deficient follicular B cells displayed higher IgD, CD40, CD44, CXCR5, and EBI2 levels, and elevated PI3K signaling upon CXCR5 stimulation. They also showed transcriptional signatures of spontaneous follicular B cell activation. This activation manifested in scattered germinal centers in situ, early plasmablasts differentiation, and signs of IgG class switch.

Details

show
hide
Language(s): eng - English
 Dates: 2021-12
 Publication Status: Published online
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000728564000001
DOI: 10.1002/JLB.1HI0621-313R
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Leukocyte Biology
  Other : J. Leukoc. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York : Liss
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 1 - 14 Identifier: ISSN: 0741-5400
CoNE: https://pure.mpg.de/cone/journals/resource/954925539169