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  Cloning, purification, crystallization and 1.57 angstrom resolution X-ray data analysis of AmsI, the tyrosine phosphatase controlling amylovoran biosynthesis in the plant pathogen Erwinia amylovora

Benini, S., Caputi, L., & Cianci, M. (2014). Cloning, purification, crystallization and 1.57 angstrom resolution X-ray data analysis of AmsI, the tyrosine phosphatase controlling amylovoran biosynthesis in the plant pathogen Erwinia amylovora. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, F70, 1693-1696. doi:10.1107/S2053230X14024947.

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EXT810.pdf (Publisher version), 329KB
 
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https://doi.org/10.1107/S2053230X14024947 (Publisher version)
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 Creators:
Benini, Stefano1, Author
Caputi, Lorenzo2, Author              
Cianci, Michele1, Author
Affiliations:
1external, ou_persistent22              
2External Organizations, ou_persistent22              

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Free keywords: ESCHERICHIA-COLI; EXOPOLYSACCHARIDE SYNTHESIS; LEVANSUCRASE; VIRULENCE; GENE; WZBBiochemistry & Molecular Biology; Biophysics; Crystallography;
 Abstract: The Gram-negative bacterium Erwinia amylovora is a destructive pathogen of plants belonging to the Rosaceae family. Amongst its pathogenicity factors, E. amylovora produces the exopolysaccharide amylovoran, which contributes to the occlusion of plant vessels, causing wilting of shoots and eventually resulting in plant death. Amylovoran biosynthesis requires the presence of 12 genes (from amsA to amsL) clustered in the ams region of the E. amylovora genome. They mostly encode glycosyl transferases (AmsG, AmsB, AmsD, AmsE, AmsJ and AmsK), proteins involved in amylovoran translocation and assembly (AmsH, AmsL and AmsC), and also a tyrosine kinase (AmsA) and a tyrosine phosphatase (AmsI), which are both involved in the regulation of amylovoran biosynthesis. The low-molecular-weight protein tyrosine phosphatase AmsI was overexpressed as a His(6)-tagged protein in Escherichia coli, purified and crystallized. X-ray diffraction data were collected to a maximum resolution of 1.57 angstrom in space group P3(1)21.

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 Dates: 2014
 Publication Status: Published in print
 Pages: 4
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1107/S2053230X14024947
Other: EXT810
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Title: Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Source Genre: Journal
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Publ. Info: Blackwell Publishing Limited
Pages: - Volume / Issue: F70 Sequence Number: - Start / End Page: 1693 - 1696 Identifier: ISSN: 1744-3091
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000017210_1