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Free keywords:
SUGAR NUCLEOTIDE; GLYCOSYLTRANSFERASES; PROBES; BIODISTRIBUTION;
GLYCOSYLATION; SPECIFICITY; SUBSTRATE; GALACTOSEBiochemistry & Molecular Biology; Pharmacology & Pharmacy; Chemistry; Sugar nucleotide; Biotransformation; Fluorosugar; Glucosyltransferase;
Natural product;
Abstract:
In connection with prospective F-18-PET imaging studies, the potential for enzymatic synthesis of fluorine-labelled glycosides of small molecules was investigated. Approaches to the enzymatic synthesis of anomeric phosphates of D-gluco-configured fluorosugars proved ineffective. In contrast, starting in the D-galacto series and relying on the consecutive action of Escherichia colt galactokinase (GalK), galactose-1-phosphate uridylyltransferase (GalPUT), uridine-5'-diphosphogalactose 4-epimerase (GalE) and oat root glucosyltransferase (SAD10), a quick and effective synthesis of 6-deoxy-6-fluoro-D-glucosyl N-methylanthranilate ester was achieved. (C) 2013 The Authors. Published by Elsevier Ltd. All rights reserved.
