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  Optical recording of light-evoked calcium signals in the functionally intact retina

Denk, W., & Detwiler, P. B. (1999). Optical recording of light-evoked calcium signals in the functionally intact retina. Proceedings of the National Academy of Sciences of the United States of America, 96(12), 7035-7040. doi:10.1073/pnas.96.12.7035.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-C7B2-D Version Permalink: https://hdl.handle.net/21.11116/0000-0009-C7B3-C
Genre: Journal Article

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 Creators:
Denk, Winfried1, Author           
Detwiler, P. B., Author
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1Bell Laboratories Lucent Technologies, Murray Hill, New Jersey, U. S. A., ou_persistent22              

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Free keywords: green fluorescent proteins synaptic transmission cells indicators rods calmodulin neurons spines fura-2 ca2+ Science & Technology - Other Topics
 Abstract: Using two-photon excitation of Fluorescent indicator dyes, we measured calcium concentration transients in retinal ganglion and amacrine cells without destroying the light sensitivity of the retina by maximally activating or bleaching the photoreceptors. This allowed an immediate assessment of the cellular morphology and study of the calcium signals evoked by visual stimuli. Calcium dynamics in individual dendritic processes could be examined for extensive periods without deterioration and with little apparent phototoxicity at excitation wavelengths of from 930 to 990 nm. Light-evoked increases in calcium were resolved in ganglion- and amacrine-cell neurites, making it possible to use optical recording to study the relationship between calcium signaling and retinal function.

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Language(s): eng - English
 Dates: 1999
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000080842200083
DOI: 10.1073/pnas.96.12.7035
ISSN: 0027-8424
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 96 (12) Sequence Number: - Start / End Page: 7035 - 7040 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230