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  Longitudinal spread of second messenger signals in isolated rod outer segments of lizards

Gray-Keller, M., Denk, W., Shraiman, B., & Detwiler, P. B. (1999). Longitudinal spread of second messenger signals in isolated rod outer segments of lizards. Journal of Physiology-London, 519(3), 679-692. doi:10.1111/j.1469-7793.1999.0679n.x.

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Gray-Keller, M., Author
Denk, Winfried1, Author           
Shraiman, B., Author
Detwiler, P. B., Author
Affiliations:
1Bell Laboratories Lucent Technologies, Murray Hill, New Jersey, U. S. A., ou_persistent22              

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Free keywords: patch-clamp recordings retinal rods diffusion-coefficient guanylate-cyclase light adaptation calcium salamander photoreceptors phosphodiesterase kinetics Neurosciences & Neurology Physiology
 Abstract: 1. In vertebrate rods activation of the phototransduction cascade by light triggers changes in the concentrations of at least two diffusible intracellular second messengers (cGMP and Ca2+) whose actions depend on how far they spread from their site of production or entry. To address questions about their spatial spread, cell-attached patch current recording and fluorescence imaging of Calcium Green-dextran were used to measure the longitudinal spread of cGMP and Ca2+, respectively, in functionally intact isolated Gecko gecko lizard rod outer segments under whole-cell voltage clamp. 2. The light-evoked changes in cGMP and Ca2+ concentrations decayed with distance from a site of steady focal activation by two-photon absorption of 1064 nm light with similar decay lengths of similar to 3.5 mu m. 3. These results can be understood on the basis of a quantitative model of coupled diffusible intracellular messengers, which is likely to have broad relevance for second messenger signalling pathways in general. 4. The decay length for the spread of adaptation from a site of steady local illumination was about 8 mu m, i.e. substantially longer than the decay lengths measured for the spread of cGMP and Ca2+. There are a number of factors, however, that could broaden the apparent relationship between functional changes in the light response and the concentration of a diffusible messenger. For these reasons the measured decay length is an upper limit estimate of the spread of adaptation and does not rule out the possibility that Ca2+ and/or cGMP carry the adaptation signal.

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Language(s): eng - English
 Dates: 1999
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000083031500006
DOI: 10.1111/j.1469-7793.1999.0679n.x
ISSN: 0022-3751
 Degree: -

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Title: Journal of Physiology-London
  Other : J. Physiol.-London
Source Genre: Journal
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Publ. Info: London : Cambridge University Press
Pages: - Volume / Issue: 519 (3) Sequence Number: - Start / End Page: 679 - 692 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_1