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  Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser

Svoboda, K., Denk, W., Knox, W. H., & Tsuda, S. (1996). Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser. Optics Letters, 21(17), 1411-1413. doi:10.1364/ol.21.001411.

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Item Permalink: https://hdl.handle.net/21.11116/0000-0009-C7A2-F Version Permalink: https://hdl.handle.net/21.11116/0000-0009-C7A3-E
Genre: Journal Article

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Svoboda, K., Author
Denk, Winfried1, Author           
Knox, W. H., Author
Tsuda, S., Author
Affiliations:
1Bell Laboratories Lucent Technologies, Murray Hill, New Jersey, U. S. A., ou_persistent22              

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Free keywords: absorber spines cells Optics
 Abstract: A Cr:LiSrAlFl laser, pumped with a diffraction-limited laser diode and mode locked with a saturable Bragg reflector, produces 90-fs pulses at 860 nm with a cw power as high as 88 mW in two beams. It is shown that this recently developed, compact, solid-state laser can be used as an excitation source for two-photon laser scanning microscopy. Morphological and functional images of neocortical and cerebellar neurons were obtained with submicrometer three-dimensional resolution. Single dendritic spines could easily be resolved deep in scattering tissue. (C) 1996 Optical Society of America

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Language(s): eng - English
 Dates: 1996
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:A1996VE31000033
DOI: 10.1364/ol.21.001411
ISSN: 0146-9592
 Degree: -

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Title: Optics Letters
Source Genre: Journal
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Publ. Info: Washington : Optical Society of America
Pages: - Volume / Issue: 21 (17) Sequence Number: - Start / End Page: 1411 - 1413 Identifier: ISSN: 0146-9592
CoNE: https://pure.mpg.de/cone/journals/resource/954925474435