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  A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits

Wilfling, F., Lee, C.-W., Erdmann, P. S., Zheng, Y., Sherpa, D., Jentsch, S., et al. (2020). A Selective Autophagy Pathway for Phase-Separated Endocytic Protein Deposits. Molecular Cell, 80(5), 764-778. doi:10.1016/j.molcel.2020.10.030.

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Genre: Journal Article

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 Creators:
Wilfling, Florian1, Author              
Lee, Chia-Wei1, Author              
Erdmann, Philipp S.2, Author              
Zheng, Yumei3, Author
Sherpa, Dawafuti4, Author              
Jentsch, Stefan1, Author              
Pfander, Boris5, Author              
Schulman, Brenda A.4, Author              
Baumeister, Wolfgang2, Author              
Affiliations:
1Jentsch, Stefan / Molecular Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565156              
2Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              
3external, ou_persistent22              
4Schulman, Brenda / Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Max Planck Society, ou_2466699              
5Pfander, Boris / DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565165              

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Free keywords: Atg11; Atg8; Ede1; clathrin-mediated endocytosis; intrinsic autophagy receptor; liquid-liquid phase separation; selective autophagy
 Abstract: Autophagy eliminates cytoplasmic content selected by autophagy receptors, which link cargo to the membrane-bound autophagosomal ubiquitin-like protein Atg8/LC3. Here, we report a selective autophagy pathway for protein condensates formed by endocytic proteins in yeast. In this pathway, the endocytic protein Ede1 functions as a selective autophagy receptor. Distinct domains within Ede1 bind Atg8 and mediate phase separation into condensates. Both properties are necessary for an Ede1-dependent autophagy pathway for endocytic proteins, which differs from regular endocytosis and does not involve other known selective autophagy receptors but requires the core autophagy machinery. Cryo-electron tomography of Ede1-containing condensates, at the plasma membrane and in autophagic bodies, shows a phase-separated compartment at the beginning and end of the Ede1-mediated selective autophagy route. Our data suggest a model for autophagic degradation of macromolecular protein complexes by the action of intrinsic autophagy receptors.

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Language(s): eng - English
 Dates: 2020-11-172020-12-03
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: We thank ... the MPIB Imaging and Biochemistry Core Facility for help with image analysis (G. Cardone and M. Spitaler) and mass spectrometry analysis (N. Nagaraj and S. Uebel) ...
 Rev. Type: Peer
 Degree: -

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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 80 (5) Sequence Number: - Start / End Page: 764 - 778 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929