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  Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes

Frei, M. S., Koch, B., Hiblot, J., & Johnsson, K. (2022). Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes. ACS Chemical Biology, 17(6), 1321-1327. doi:10.1021/acschembio.2c00041.

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Frei, Michelle S.1, Author           
Koch, Birgit1, Author           
Hiblot, Julien1, Author           
Johnsson, Kai1, Author           
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1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: Fluorescence lifetime multiplexing requires fluorescent probes with distinct fluorescence lifetimes but similar spectral properties. Even though synthetic probes for many cellular targets are available for multicolor live-cell fluorescence microscopy, few of them have been characterized for their use in fluorescence lifetime multiplexing. Here we demonstrate that from a panel of 18 synthetic probes, eight pairwise combinations are suitable for fluorescence lifetime multiplexing in living mammalian cell lines. Moreover, combining multiple pairs in different spectral channels enables us to image up to six different biological targets, effectively doubling the number of observable targets. The combination of synthetic probes with fluorescence lifetime multiplexing is thus a powerful approach for live-cell imaging.

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Language(s): eng - English
 Dates: 2022-01-152022-05-122022-05-182022-06-17
 Publication Status: Issued
 Pages: 7
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 Rev. Type: Peer
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Title: ACS Chemical Biology
  Abbreviation : ACS Chem. Biol.
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 17 (6) Sequence Number: - Start / End Page: 1321 - 1327 Identifier: ISSN: 1554-8929
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000035040