English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes

Frei, M. S., Koch, B., Hiblot, J., & Johnsson, K. (2022). Live-cell fluorescence lifetime multiplexing using synthetic fluorescent probes. ACS Chemical Biology, 17(6), 1321-1327. doi:10.1021/acschembio.2c00041.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
ACSChemBiol_17_2022_1321.pdf (Any fulltext), 7MB
 
File Permalink:
-
Name:
ACSChemBiol_17_2022_1321.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ACSChemBiol_17_2022_1321_Suppl.pdf (Supplementary material), 3MB
 
File Permalink:
-
Name:
ACSChemBiol_17_2022_1321_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Frei, Michelle S.1, Author              
Koch, Birgit1, Author              
Hiblot, Julien1, Author              
Johnsson, Kai1, Author              
Affiliations:
1Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

Content

show
hide
Free keywords: -
 Abstract: Fluorescence lifetime multiplexing requires fluorescent probes with distinct fluorescence lifetimes but similar spectral properties. Even though synthetic probes for many cellular targets are available for multicolor live-cell fluorescence microscopy, few of them have been characterized for their use in fluorescence lifetime multiplexing. Here we demonstrate that from a panel of 18 synthetic probes, eight pairwise combinations are suitable for fluorescence lifetime multiplexing in living mammalian cell lines. Moreover, combining multiple pairs in different spectral channels enables us to image up to six different biological targets, effectively doubling the number of observable targets. The combination of synthetic probes with fluorescence lifetime multiplexing is thus a powerful approach for live-cell imaging.

Details

show
hide
Language(s): eng - English
 Dates: 2022-01-152022-05-122022-05-182022-06-17
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Chemical Biology
  Abbreviation : ACS Chem. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 17 (6) Sequence Number: - Start / End Page: 1321 - 1327 Identifier: ISSN: 1554-8929
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000035040