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  Imaging keratan sulfate in ocular tissue sections by immunofluorescence microscopy and LA-ICP-MS

Boyraz, B., Saatz, J., Pompös, I.-M., Gad, M., Dernedde, J., Maier, A.-K.-B., et al. (2022). Imaging keratan sulfate in ocular tissue sections by immunofluorescence microscopy and LA-ICP-MS. ACS applied bio materials, 5(2), 853-861. doi:10.1021/acsabm.1c01240.

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 Creators:
Boyraz, Burak, Author
Saatz, Jessica, Author
Pompös, Inga-Marie, Author
Gad, Michel, Author
Dernedde, Jens, Author
Maier, Anna-Karina B., Author
Moscovitz, Oren1, Author              
Seeberger, Peter H.2, Author              
Traub, Heike, Author
Tauber, Rudolf, Author
Affiliations:
1Oren Moscovitz, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3176803              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              

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Free keywords: extracellular matrix; glycosaminoglycans; keratan sulfate; ICP-MS; immunofluorescence; immunohistochemistry; imaging; cornea
 Abstract: Carbohydrate-specific antibodies can serve as valuable tools to monitor alterations in the extracellular matrix resulting from pathologies. Here, the keratan sulfate-specific monoclonal antibody MZ15 was characterized in more detail by immunofluorescence microscopy as well as laser ablation ICP-MS using tissue cryosections and paraffin-embedded samples. Pretreatment with keratanase II prevented staining of samples and therefore demonstrated efficient enzymatic keratan sulfate degradation. Random fluorescent labeling and site-directed introduction of a metal cage into MZ15 were successful and allowed for a highly sensitive detection of the keratan sulfate landscape in the corneal stroma from rats and human tissue.

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Language(s): eng - English
 Dates: 2022-01-252022
 Publication Status: Published in print
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 Identifiers: DOI: 10.1021/acsabm.1c01240
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Title: ACS applied bio materials
  Other : ACS Appl. Bio Mater.
Source Genre: Journal
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Publ. Info: Washington, DC : ACS Publications
Pages: - Volume / Issue: 5 (2) Sequence Number: - Start / End Page: 853 - 861 Identifier: ISSN: 2576-6422