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  Expression of Lineage Transcription Factors Identifies Differences in Transition States of Induced Human Oligodendrocyte Differentiation

Raabe, F. J., Stephan, M., Waldeck, J. B., Huber, V., Demetriou, D., Kannaiyan, N., et al. (2011). Expression of Lineage Transcription Factors Identifies Differences in Transition States of Induced Human Oligodendrocyte Differentiation. Cells, 11(2): (2):241. doi:10.3390/cells11020241.

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cells_Raabe et al_2022.pdf (Publisher version), 6MB
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© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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 Creators:
Raabe, Florian J. , Author
Stephan, Marius , Author
Waldeck, Jan Benedikt , Author
Huber, Verena, Author
Demetriou, Damianos, Author
Kannaiyan, Nirmal , Author
Galinski, Sabrina , Author
Glaser, Laura V.1, Author              
Wehr, Michael C. , Author
Ziller, Michael J. , Author
Schmitt, Andrea, Author
Falkai, Peter, Author
Rossner, Moritz J. , Author
Affiliations:
1Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              

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Free keywords: RNA velocity; directed differentiation; hiPSC; human pluripotent stem cells; oligodendrocytes; scRNAseq
 Abstract: Oligodendrocytes (OLs) are critical for myelination and are implicated in several brain disorders. Directed differentiation of human-induced OLs (iOLs) from pluripotent stem cells can be achieved by forced expression of different combinations of the transcription factors SOX10 (S), OLIG2 (O), and NKX6.2 (N). Here, we applied quantitative image analysis and single-cell transcriptomics to compare different transcription factor (TF) combinations for their efficacy towards robust OL lineage conversion. Compared with S alone, the combination of SON increases the number of iOLs and generates iOLs with a more complex morphology and higher expression levels of myelin-marker genes. RNA velocity analysis of individual cells reveals that S generates a population of oligodendrocyte-precursor cells (OPCs) that appear to be more immature than those generated by SON and to display distinct molecular properties. Our work highlights that TFs for generating iOPCs or iOLs should be chosen depending on the intended application or research question, and that SON might be beneficial to study more mature iOLs while S might be better suited to investigate iOPC biology.

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Language(s): eng - English
 Dates: 2022-01-072011-01-11
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.3390/cells11020241
PMID: 35053357
 Degree: -

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Title: Cells
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI
Pages: - Volume / Issue: 11 (2) Sequence Number: (2):241 Start / End Page: - Identifier: CoNE: https://pure.mpg.de/cone/journals/resource/2073-4409