English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Emergence and patterning dynamics of mouse-definitive endoderm

Pour, M., Sampath Kumar, A., Farag, N., Bolondi, A., Kretzmer, H., Walther, M., et al. (2022). Emergence and patterning dynamics of mouse-definitive endoderm. iScience, 25(1): 103556. doi:10.1016/j.isci.2021.103556.

Item is

Files

show Files
hide Files
:
iScience_Pour et al_2022.pdf (Publisher version), 6MB
Name:
iScience_Pour et al_2022.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
© 2021 The Authors

Locators

show

Creators

show
hide
 Creators:
Pour, Maayan , Author
Sampath Kumar, Abhishek1, Author           
Farag, Naama , Author
Bolondi, Adriano1, Author           
Kretzmer, Helene1, Author           
Walther, Maria1, Author           
Wittler, Lars2, Author           
Meissner, Alexander1, Author           
Nachman, Iftach , Author
Affiliations:
1Dept. of Genome Regulation (Head: Alexander Meissner), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2379694              
2Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

Content

show
hide
Free keywords: Developmental genetics; Embryology
 Abstract: The segregation of definitive endoderm (DE) from bipotent mesendoderm progenitors leads to the formation of two distinct germ layers. Dissecting DE commitment and onset has been challenging as it occurs within a narrow spatiotemporal window in the embryo. Here, we employ a dual Bra/Sox17 reporter cell line to study DE onset dynamics. We find Sox17 expression initiates in vivo in isolated cells within a temporally restricted window. In 2D and 3D in vitro models, DE cells emerge from mesendoderm progenitors at a temporally regular, but spatially stochastic pattern, which is subsequently arranged by self-sorting of Sox17 + cells. A subpopulation of Bra-high cells commits to a Sox17+ fate independent of external Wnt signal. Self-sorting coincides with upregulation of E-cadherin but is not necessary for DE differentiation or proliferation. Our in vivo and in vitro results highlight basic rules governing DE onset and patterning through the commonalities and differences between these systems.

Details

show
hide
Language(s): eng - English
 Dates: 2021-12-012022-01-21
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.isci.2021.103556
PMID: 34988400
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: iScience
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis : Elsevier
Pages: - Volume / Issue: 25 (1) Sequence Number: 103556 Start / End Page: - Identifier: ISSN: 2589-0042
CoNE: https://pure.mpg.de/cone/journals/resource/2589-0042