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  Emergence and patterning dynamics of mouse-definitive endoderm

Pour, M., Sampath Kumar, A., Farag, N., Bolondi, A., Kretzmer, H., Walther, M., et al. (2022). Emergence and patterning dynamics of mouse-definitive endoderm. iScience, 25(1): (1):103556. doi:10.1016/j.isci.2021.103556.

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© 2021 The Authors

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 Creators:
Pour, Maayan , Author
Sampath Kumar, Abhishek1, Author              
Farag, Naama , Author
Bolondi, Adriano1, Author              
Kretzmer, Helene1, Author              
Walther, Maria1, Author              
Wittler, Lars2, Author              
Meissner, Alexander1, Author              
Nachman, Iftach , Author
Affiliations:
1Dept. of Genome Regulation (Head: Alexander Meissner), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2379694              
2Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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Free keywords: Developmental genetics; Embryology
 Abstract: The segregation of definitive endoderm (DE) from bipotent mesendoderm progenitors leads to the formation of two distinct germ layers. Dissecting DE commitment and onset has been challenging as it occurs within a narrow spatiotemporal window in the embryo. Here, we employ a dual Bra/Sox17 reporter cell line to study DE onset dynamics. We find Sox17 expression initiates in vivo in isolated cells within a temporally restricted window. In 2D and 3D in vitro models, DE cells emerge from mesendoderm progenitors at a temporally regular, but spatially stochastic pattern, which is subsequently arranged by self-sorting of Sox17 + cells. A subpopulation of Bra-high cells commits to a Sox17+ fate independent of external Wnt signal. Self-sorting coincides with upregulation of E-cadherin but is not necessary for DE differentiation or proliferation. Our in vivo and in vitro results highlight basic rules governing DE onset and patterning through the commonalities and differences between these systems.

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Language(s): eng - English
 Dates: 2021-12-012022-01-21
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.isci.2021.103556
PMID: 34988400
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Title: iScience
Source Genre: Journal
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Publ. Info: Amsterdam ; Bosten ; London ; New York ; Oxford ; Paris ; Philadelphia ; San Diego ; St. Louis : Elsevier
Pages: - Volume / Issue: 25 (1) Sequence Number: (1):103556 Start / End Page: - Identifier: ISSN: 2589-0042
CoNE: https://pure.mpg.de/cone/journals/resource/2589-0042