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  Production of levan from Bacillus subtilis var. natto and apoptotic effect on SH-SY5Y neuroblastoma cells

Vieira, A. M., Zahed, F., Crispim, A. C., de Souza Bento, E., de Freitas Oliveira França, R., Pinheiro, I. O., et al. (2021). Production of levan from Bacillus subtilis var. natto and apoptotic effect on SH-SY5Y neuroblastoma cells. Carbohydrate Polymers, 273: 118613. doi:10.1016/j.carbpol.2021.118613.

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 Creators:
Vieira, A. M., Author
Zahed, F.1, Author           
Crispim, A. C., Author
de Souza Bento, E., Author
de Freitas Oliveira França, R. , Author
Pinheiro, I. O., Author
Pardo, L. A.1, Author           
Carvalho, B. M., Author
Affiliations:
1Oncophysiology, Molecular biology of neuronal signals, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173657              

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Free keywords: Exopolysaccharide; Neoplastic cells; Live-cell analysis
 Abstract: Levan is a high-valued polysaccharide of fructose produced by several microbial species. These polysaccharides have been described as effective therapeutic agents in some human disease conditions, such as cancer, heart diseases and diabetes. The objective of this study was to examine the effect of levan (β-(2 → 6)-fructan) produced through sucrose fermentation by B. subtilis var. natto on the proliferation rate, cytotoxicity, and apoptosis of human neuroblastoma SH-SY5Y cells. It was obtained 41.44 g/L of levan in 18 h by biotechnological fermentation and SH-SY5Y cells were exposed to 1000 μg/mL of levan. The treatment with 1000 μg/mL of levan induced apoptosis in SH-SY5Y cancer cells by the significant increase in Annexin V/7-AAD and caspase 3/7 activation, but did not decrease proliferation or triggered a cytotoxic effect. 1000 μg/mL levan treatment is a promising therapeutic strategy for SH-SY5Y neuroblastoma cells.

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Language(s): eng - English
 Dates: 2021-08-272021-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.carbpol.2021.118613
 Degree: -

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Title: Carbohydrate Polymers
Source Genre: Journal
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Pages: - Volume / Issue: 273 Sequence Number: 118613 Start / End Page: - Identifier: -