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  A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain

Bosch-Queralt, M., Tiwari, V., Damkou, A., Vaculciakova, L., Alexopoulos, I., & Simons, M. (2022). A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain. STAR Protocols, 3(1): 101141. doi:10.1016/j.xpro.2022.101141.

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Bosch-Queralt, Mar1, 2, Author
Tiwari, Vini1, 2, Author
Damkou, Alkmini1, 2, Author
Vaculciakova, Lenka3, Author              
Alexopoulos, Ioannis1, 2, Author
Simons, Mikael1, 2, 4, Author
Affiliations:
1Institute of Neuronal Cell Biology, TU Munich, Germany, ou_persistent22              
2German Center for Neurodegenerative Diseases, Munich, Germany, ou_persistent22              
3Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              
4Munich Cluster for Systems Neurology (SyNergy), Ludwig Maximilians University Munich, Germany, ou_persistent22              

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 Abstract: Lysolecithin injections into the white matter tracts of the central nervous system are a valuable tool to study remyelination, but evaluating the resulting demyelinating lesion size is challenging. Here, we present a protocol to consistently measure the volume of demyelination and remyelination in mice following brain lysolecithin injections. We describe serial sectioning of the lesion, followed by the evaluation of the demyelinated area in two-dimensional images. We then detail the computation of the volume using our own automated iPython script.

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Language(s): eng - English
 Dates: 2022-01-292022-03-18
 Publication Status: Published in print
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 Identifiers: DOI: 10.1016/j.xpro.2022.101141
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Grant ID : TRR128-2, TRR 274, EXC2145
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Funding organization : German Research Foundation

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Title: STAR Protocols
Source Genre: Journal
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Publ. Info: Cambridge, MA ; Amsterdam : Cell Press ; Elsevier
Pages: - Volume / Issue: 3 (1) Sequence Number: 101141 Start / End Page: - Identifier: ISSN: 2666-1667
CoNE: https://pure.mpg.de/cone/journals/resource/2666-1667