ausblenden:
Schlagwörter:
Animals
Cyclins/physiology
Electric Conductivity
Ether-A-Go-Go Potassium Channels
Ion Channel Gating
Maturation-Promoting Factor/*physiology
Membrane Potentials
Oocytes
Potassium Channels/*physiology
Progesterone/pharmacology
Signal Transduction
Spermine/pharmacology
Time Factors
Xenopus laevis
Zusammenfassung:
The cell cycle is the crucial process that leads to mitosis in all cell types. The dramatic redirectioning of many cellular processes during the cycle is known to involve ion channels, either changing their level of expression or their voltage dependence, as in the case of inward rectifiers. Here we describe the specific inhibition of heterologously expressed ionic channels at the onset of maturation in Xenopus oocytes. In cells expressing rat eag (R-eag) potassium channels, maturation induces a dramatic reduction in the current amplitude, which is almost complete in most cases. The key molecule in oocyte maturation, the mitosis-promoting factor (a complex of cyclin B and p34cdc2), is able to induce similar changes when injected into the oocytes.
