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  High iron and iron household protein contents in perineuronal net-ensheathed neurons ensure energy metabolism with safe iron handling

Reinert, A., Reinert, T., Arendt, T., & Morawski, M. (2022). High iron and iron household protein contents in perineuronal net-ensheathed neurons ensure energy metabolism with safe iron handling. International Journal of Molecular Sciences, 23(3): 1634. doi:10.3390/ijms23031634.

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 Creators:
Reinert, Anja1, 2, Author
Reinert, Tilo2, 3, Author              
Arendt, Thomas2, Author
Morawski, Markus2, 3, Author              
Affiliations:
1Institute of Anatomy, Histology and Embryology, University of Leipzig, Germany, ou_persistent22              
2Paul Flechsig Institute for Brain Research, University of Leipzig, Germany, ou_persistent22              
3Department Neurophysics (Weiskopf), MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_2205649              

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Free keywords: Brain; Iron; Iron proteins; Perineuronal net; Neurodegeneration; Transferrin; Transferrin receptor; Ferritin H/L; DMT1; MTP1; Cellular quantification
 Abstract: A subpopulation of neurons is less vulnerable against iron-induced oxidative stress and neurodegeneration. A key feature of these neurons is a special extracellular matrix composition that forms a perineuronal net (PN). The PN has a high affinity to iron, which suggests an adapted iron sequestration and metabolism of the ensheathed neurons. Highly active, fast-firing neurons—which are often ensheathed by a PN—have a particular high metabolic demand, and therefore may have a higher need in iron. We hypothesize that PN-ensheathed neurons have a higher intracellular iron concentration and increased levels of iron proteins. Thus, analyses of cellular and regional iron and the iron proteins transferrin (Tf), Tf receptor 1 (TfR), ferritin H/L (FtH/FtL), metal transport protein 1 (MTP1 aka ferroportin), and divalent metal transporter 1 (DMT1) were performed on Wistar rats in the parietal cortex (PC), subiculum (SUB), red nucleus (RN), and substantia nigra (SNpr/SNpc). Neurons with a PN (PN+) have higher iron concentrations than neurons without a PN: PC 0.69 mM vs. 0.51 mM, SUB 0.84 mM vs. 0.69 mM, SN 0.71 mM vs. 0.63 mM (SNpr)/0.45 mM (SNpc). Intracellular Tf, TfR and MTP1 contents of PN+ neurons were consistently increased. The iron concentration of the PN itself is not increased. We also determined the percentage of PN+ neurons: PC 4%, SUB 5%, SNpr 45%, RN 86%. We conclude that PN+ neurons constitute a subpopulation of resilient pacemaker neurons characterized by a bustling iron metabolism and outstanding iron handling capabilities. These properties could contribute to the low vulnerability of PN+ neurons against iron-induced oxidative stress and degeneration.

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Language(s): eng - English
 Dates: 2022-01-242021-12-212022-01-282022-01-31
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.3390/ijms23031634
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Title: International Journal of Molecular Sciences
  Abbreviation : Int. J. Mol. Sci.
Source Genre: Journal
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Publ. Info: Basel, Switzerland : MDPI AG
Pages: - Volume / Issue: 23 (3) Sequence Number: 1634 Start / End Page: - Identifier: ISSN: 1422-0067
CoNE: https://pure.mpg.de/cone/journals/resource/1422-0067