English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Wnt/beta-catenin signaling promotes neurogenesis in the diencephalospinal dopaminergic system of embryonic zebrafish

Westphal, M., Panza, P., Kastenhuber, E., Wehrle, J., & Driever, W. (2022). Wnt/beta-catenin signaling promotes neurogenesis in the diencephalospinal dopaminergic system of embryonic zebrafish. SCIENTIFIC REPORTS, 12(1): 1030. doi:10.1038/s41598-022-04833-8.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Westphal, Markus, Author
Panza, Paolo1, Author              
Kastenhuber, Edda, Author
Wehrle, Johanna, Author
Driever, Wolfgang, Author
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

Content

show
hide
Free keywords: -
 Abstract: Wnt/beta-catenin signaling contributes to patterning, proliferation, and differentiation throughout vertebrate neural development. Wnt/beta-catenin signaling is important for mammalian midbrain dopaminergic neurogenesis, while little is known about its role in ventral forebrain dopaminergic development. Here, we focus on the A11-like, Otp-dependent diencephalospinal dopaminergic system in zebrafish. We show that Wnt ligands, receptors and extracellular antagonist genes are expressed in the vicinity of developing Otp-dependent dopaminergic neurons. Using transgenic Wnt/beta-catenin-reporters, we found that Wnt/beta-catenin signaling activity is absent from these dopaminergic neurons, but detected Wnt/beta-catenin activity in cells adjacent to the caudal DC5/6 clusters of Otp-dependent dopaminergic neurons. Pharmacological manipulations of Wnt/beta-catenin signaling activity, as well as heat-shock driven overexpression of Wnt agonists and antagonists, interfere with the development of DC5/6 dopaminergic neurons, such that Wnt/beta-catenin activity positively correlates with their number. Wnt/beta-catenin activity promoted dopaminergic development specifically at stages when DC5/6 dopaminergic progenitors are in a proliferative state. Our data suggest that Wnt/beta-catenin signaling acts in a spatially and temporally restricted manner on proliferative dopaminergic progenitors in the hypothalamus to positively regulate the size of the dopaminergic neuron groups DC5 and DC6.

Details

show
hide
Language(s):
 Dates: 2022-01-19
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000744620600006
DOI: 10.1038/s41598-022-04833-8
PMID: 35046434
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: SCIENTIFIC REPORTS
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 12 (1) Sequence Number: 1030 Start / End Page: - Identifier: ISSN: 2045-2322