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  Ancient DNA methods improve forensic DNA profiling of Korean War and World War II unknowns

Zavala, E. I., Thomas, J. T., Sturk-Andreaggi, K., Daniels-Higginbotham, J., Meyers, K. K., Barrit-Ross, S., et al. (2022). Ancient DNA methods improve forensic DNA profiling of Korean War and World War II unknowns. Genes, 13(129). doi:10.3390/genes13010129.

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Zavala_Ancient_Genes_2022.pdf (Publisher version), 4MB
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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).
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Zavala_Ancient_Genes_Suppl_2022.zip (Supplementary material), 307KB
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Zavala_Ancient_Genes_Suppl_2022.zip
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Gold
Visibility:
Public
MIME-Type / Checksum:
application/zip / [MD5]
Technical Metadata:
Copyright Date:
2022
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© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

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 Creators:
Zavala, Elena I.1, 2, Author                 
Thomas, Jacqueline Tyler, Author
Sturk-Andreaggi, Kimberly, Author
Daniels-Higginbotham, Jennifer, Author
Meyers, Kerriann K., Author
Barrit-Ross, Suzanne, Author
Ayinuer-Petri, Aximu1, Author           
Richter, Julia1, Author           
Nickel, Birgit3, Author           
Berg, Gregory E., Author
McMahon, Timothy P., Author
Meyer, Matthias1, Author           
Marshall, Charla, Author
Affiliations:
1Advanced DNA Sequencing Techniques, Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_2074332              
2The Leipzig School of Human Origins (IMPRS), Max Planck Institute for Evolutionary Anthropology, Max Planck Society, Deutscher Platz 6, 04103 Leipzig, DE, ou_1497688              
3Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Max Planck Society, ou_1497672              

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Free keywords: degraded DNA, massively parallel sequencing (MPS), mitochondrial DNA, forensic DNA profiling, ancient DNA, human identification
 Abstract: The integration of massively parallel sequencing (MPS) technology into forensic casework has been of particular benefit to the identification of unknown military service members. However, highly degraded or chemically treated skeletal remains often fail to provide usable DNA profiles, even with sensitive mitochondrial (mt) DNA capture and MPS methods. In parallel, the ancient DNA field has developed workflows specifically for degraded DNA, resulting in the successful recovery of nuclear DNA and mtDNA from skeletal remains as well as sediment over 100,000 years old. In this study we use a set of disinterred skeletal remains from the Korean War and World War II to test if ancient DNA extraction and library preparation methods improve forensic DNA profiling. We identified an ancient DNA extraction protocol that resulted in the recovery of significantly more human mtDNA fragments than protocols previously used in casework. In addition, utilizing single-stranded rather than double-stranded library preparation resulted in increased attainment of reportable mtDNA profiles. This study emphasizes that the combination of ancient DNA extraction and library preparation methods evaluated here increases the success rate of DNA profiling, and likelihood of identifying historical remains.

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Language(s): eng - English
 Dates: 2022-01-11
 Publication Status: Published online
 Pages: 19
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.3390/genes13010129
 Degree: -

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Title: Genes
Source Genre: Journal
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Publ. Info: Basel : MDPI
Pages: - Volume / Issue: 13 (129) Sequence Number: - Start / End Page: - Identifier: ISSN: 2073-4425