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  Breaking a species barrier by enabling hybrid recombination

Bozdag, G. O., Ono, J., Denton, J. A., Karakoc, E., Hunter, N., Leu, J.-Y., et al. (2021). Breaking a species barrier by enabling hybrid recombination. Current Biology, 31(4), R180-R181. doi:10.1016/j.cub.2020.12.038.

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 Creators:
Bozdag, G. Ozan1, 2, Author           
Ono, Jasmine, Author
Denton, Jai A.2, Author           
Karakoc, Emre2, Author           
Hunter, Neil, Author
Leu, Jun-Yi, Author
Greig, Duncan1, 2, Author           
Affiliations:
1Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445640              
2External Organizations, ou_persistent22              

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 Abstract: Summary Hybrid sterility maintains reproductive isolation between species by preventing them from exchanging genetic material1. Anti-recombination can contribute to hybrid sterility when different species’ chromosome sequences are too diverged to cross over efficiently during hybrid meiosis, resulting in chromosome mis-segregation and aneuploidy. The genome sequences of the yeasts Saccharomyces cerevisiae and Saccharomyces paradoxus have diverged by about 12% and their hybrids are sexually sterile: nearly all of their gametes are aneuploid and inviable. Previous methods to increase hybrid yeast fertility have targeted the anti-recombination machinery by enhancing meiotic crossing over. However, these methods also have counteracting detrimental effects on gamete viability due to increased mutagenesis2 and ectopic recombination3. Therefore, the role of anti-recombination has not been fully revealed, and it is often dismissed as a minor player in speciation1. By repressing two genes, SGS1 and MSH2, specifically during meiosis whilst maintaining their mitotic expression, we were able to increase hybrid fertility 70-fold, to the level of non-hybrid crosses, confirming that anti-recombination is the principal cause of hybrid sterility. Breaking this species barrier allows us to generate, for the first time, viable euploid gametes containing recombinant hybrid genomes from these two highly diverged parent species.

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Language(s): eng - English
 Dates: 2021-02-222021
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.cub.2020.12.038
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Title: Current Biology
  Abbreviation : Curr. Biol.
Source Genre: Journal
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Publ. Info: London, UK : Cell Press
Pages: - Volume / Issue: 31 (4) Sequence Number: - Start / End Page: R180 - R181 Identifier: ISSN: 0960-9822
CoNE: https://pure.mpg.de/cone/journals/resource/954925579107