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  Evolutionary flexibility in routes to mat formation by Pseudomonas

Mukherjee, A., Dechow-Seligmann, G., & Gallie, J. (2022). Evolutionary flexibility in routes to mat formation by Pseudomonas. Molecular Microbiology, 117(2), 394-410. doi:10.1111/mmi.14855.

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Molecular Microbiology - 2021 - Mukherjee - Evolutionary flexibility in routes to mat formation by Pseudomonas.pdf (Publisher version), 3MB
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Molecular Microbiology - 2021 - Mukherjee - Evolutionary flexibility in routes to mat formation by Pseudomonas.pdf
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Mukherjee, Anuradha1, Author              
Dechow-Seligmann, Gunda2, Author              
Gallie, Jenna2, Author              
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1External Organizations, ou_persistent22              
2Research Group Microbial Evolutionary Dynamics, Department Evolutionary Theory, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_2253646              

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Free keywords: biofilms, cellulose, exopolysaccharides, Pel, Psl
 Abstract: Abstract Many bacteria form mats at the air-liquid interface of static microcosms. These structures typically involve the secretion of exopolysaccharides, the production of which is often controlled by the secondary messenger c-di-GMP. Mechanisms of mat formation have been particularly well characterized in Pseudomonas fluorescens SBW25; stimuli or mutations that increase c-di-GMP production by diguanylate cyclases (WspR, AwsR, and MwsR) result in the secretion of cellulose and mat formation. Here, we characterize and compare mat formation in two close relatives of SBW25: Pseudomonas simiae PICF7 and P. fluorescens A506. We find that PICF7?the strain more closely related to SBW25?can form mats through mutations affecting the activity of the same three diguanylate cyclases as SBW25. However, instead of cellulose, these mutations activate production of the exopolysaccharide Pel. We also provide evidence for at least two further?as yet uncharacterized?routes to mat formation by PICF7. P. fluorescens A506, while retaining the same mutational routes to mat formation as SBW25 and PICF7, preferentially forms mats by a semi-heritable mechanism that culminates in Psl and Pga over-production. Our results demonstrate a high level of evolutionary flexibility in the molecular and structural routes to mat formation, even among close relatives.

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Language(s): eng - English
 Dates: 2021-11-302021-08-062021-11-302022-02
 Publication Status: Published in print
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 Identifiers: DOI: 10.1111/mmi.14855
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Title: Molecular Microbiology
  Other : Mol. Microbiol.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 117 (2) Sequence Number: - Start / End Page: 394 - 410 Identifier: ISSN: 0950-382X
CoNE: https://pure.mpg.de/cone/journals/resource/954925574950