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  A genetically encoded sensor for in vivo imaging of orexin neuropeptides

Duffet, L., Kosar, S., Panniello, M., Viberti, B., Bracey, E., Zych, A. D., et al. (2022). A genetically encoded sensor for in vivo imaging of orexin neuropeptides. Nature Methods, 19(2), 231-241. doi:10.1038/s41592-021-01390-2.

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 Creators:
Duffet, Loic, Author
Kosar, Seher, Author
Panniello, Mariangela, Author
Viberti, Bianca, Author
Bracey, Edward, Author
Zych, Anna D.1, 2, Author              
Radoux-Mergault, Arthur, Author
Zhou, Xuehan, Author
Dernic, Jan, Author
Ravotto, Luca, Author
Tsai, Yuan-Chen, Author
Figueiredo, Marta, Author
Tyagarajan, Shiva K., Author
Weber, Bruno, Author
Stoeber, Miriam, Author
Gogolla, Nadine, Author
Schmidt, Markus H., Author
Adamantidis, Antoine R., Author
Fellin, Tommaso, Author
Burdakov, Denis, Author
Patriarchi, Tommaso, Author more..
Affiliations:
1IMPRS Translational Psychiatry, Max Planck Institute of Psychiatry, Max Planck Society, ou_3318616              
2Max Planck Research Group: Circuits for Emotion / Gogolla, MPI of Neurobiology, Max Planck Society, ou_2054290              

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Free keywords: Index Medicus
 Abstract: Orexins (also called hypocretins) are hypothalamic neuropeptides that carry out essential functions in the central nervous system; however, little is known about their release and range of action in vivo owing to the limited resolution of current detection technologies. Here we developed a genetically encoded orexin sensor (OxLight1) based on the engineering of circularly permutated green fluorescent protein into the human type-2 orexin receptor. In mice OxLight1 detects optogenetically evoked release of endogenous orexins in vivo with high sensitivity. Photometry recordings of OxLight1 in mice show rapid orexin release associated with spontaneous running behavior, acute stress and sleep-to-wake transitions in different brain areas. Moreover, two-photon imaging of OxLight1 reveals orexin release in layer 2/3 of the mouse somatosensory cortex during emergence from anesthesia. Thus, OxLight1 enables sensitive and direct optical detection of orexin neuropeptides with high spatiotemporal resolution in living animals. © 2022. The Author(s).

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Language(s): eng - English
 Dates: 2022-02-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: MEDLINE:35145320
DOI: 10.1038/s41592-021-01390-2
ISSN: 1548-7105
 Degree: -

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Title: Nature Methods
  Other : Nature Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Nature Publishing Group
Pages: - Volume / Issue: 19 (2) Sequence Number: - Start / End Page: 231 - 241 Identifier: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556